2026-04-18T00:18:34Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/8832025-10-22T11:25:02Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc León, Maela author Berbegal, Mónica author Rodríguez-Reina, José M. author Elena, Georgina author Abad-Campos, Paloma author Ramón-Albalat, Antonio author OImo, Diego author Vicent, Antonio author Luque, Jordi author Miarnau, Xavier author Agustí-Brisach, Carlos author Trapero, Antonio author Capote, Nieves author Arroyo, Francisco T. author Avilés, Manuel author Gramaje, David author Andrés-Sodupe, Marcos author Armengol, Josep author 2020-07-23 Two hundred and twenty-five Diaporthe isolates were collected from 2005 to 2019 in almond orchards showing twig cankers and shoot blight symptoms in five different regions across Spain. Multilocus DNA sequence analysis with five loci (ITS, tub, tef-1α, cal and his), allowed the identification of four known Diaporthe species, namely: D. amygdali, D. eres, D. foeniculina and D. phaseolorum. Moreover, a novel phylogenetic species, D. mediterranea, was described. Diaporthe amygdali was the most prevalent species, due to the largest number of isolates (85.3%) obtained from all sampled regions. The second most frequent species was D. foeniculina (10.2%), followed by D. mediterranea (3.6%), D. eres and D. phaseolorum, each with only one isolate. Pathogenicity tests were performed using one-year-old almond twigs cv. Vayro and representative isolates of the different species. Except for D. foeniculina and D. phaseolorum, all Diaporthe species were able to cause lesions significantly different from those developed on the uninoculated controls. Diaporthe mediterranea caused the most severe symptoms. These results confirm D. amygdali as a key pathogen of almonds in Spain. Moreover, the new species, D. mediterranea, should also be considered as a potential important causal agent of twig cankers and shoot blight on this crop León, Maela, Mónica Berbegal, José M. Rodríguez-Reina, Georgina Elena, Paloma Abad-Campos, Antonio Ramón-Albalat, and Diego Olmo et al. 2020. "Identification And Characterization Of Diaporthe Spp. Associated With Twig Cankers And Shoot Blight Of Almonds In Spain". Agronomy 10 (8): 1062. doi:10.3390/agronomy10081062. 2073-4395 http://hdl.handle.net/20.500.12327/883 https://doi.org/10.3390/agronomy10081062 Identification and characterization of Diaporthe spp. associated with twig cankers and shoot blight of almonds in Spain