2026-04-13T00:08:50Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/8762025-10-22T11:18:56Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Parés, Sílvia author Fàbregas, Francesc author Bach, Àlex author Garcia-Fruitos, Elena author de Prado, Ana author Alomar, Oscar author 2020-02-11 Mammary serum amyloid A3 (M-SAA3) has shown potential in stimulating innate immunity during intramammary infections, at calving and at dryoff. In this study, we produced recombinant caprine M-SAA3 to test its ability to reduce intramammary infections with Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, and Escherichia coli, which are all common mastitis-producing pathogens. Recombinant production of M-SAA3 (followed by lipopolysaccharide removal to avoid lipopolysaccharide-nonspecific stimulation of the immune system) was successfully achieved. Mammary serum amyloid A3 stimulated the expression of IL-8 in a dose-dependent manner in primary mammary cultures. Although a direct killing effect on Staph. aureus by M-SAA3 was not detected, this acute phase protein was able to reduce Staph. aureus, Strep. uberis, and Strep. dysgalactiae infections by up to 50% and induced a reduction in E. coli counts of 67%. In general, the best concentration of caprine M-SAA3 for inhibiting infections was the lowest concentration tested (10 μg/mL), although higher concentrations (up to 160 μg/mL) increased its antimicrobial potential against some pathogens. Parés, Sílvia, Francesc Fàbregas, Àlex Bach, Elena Garcia-Fruitós, Ana de Prado, and Anna Arís. 2020. "Short Communication: Recombinant Mammary Serum Amyloid A3 As A Potential Strategy For Preventing Intramammary Infections In Dairy Cows At Dryoff". Journal Of Dairy Science 103 (4): 3615-3621. doi:10.3168/jds.2019-17276. 0022-0302 http://hdl.handle.net/20.500.12327/876 https://doi.org/10.3168/jds.2019-17276 Short communication: Recombinant mammary serum amyloid A3 as a potential strategy for preventing intramammary infections in dairy cows at dryoff