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2025-10-22T11:18:56Z urn:hdl:20.500.12327/876 Short communication: Recombinant mammary serum amyloid A3 as a potential strategy for preventing intramammary infections in dairy cows at dryoff Parés, Sílvia Fàbregas, Francesc Bach, Àlex Garcia-Fruitos, Elena de Prado, Ana Alomar, Oscar Producció Animal Producció de Remugants Mammary serum amyloid A3 (M-SAA3) has shown potential in stimulating innate immunity during intramammary infections, at calving and at dryoff. In this study, we produced recombinant caprine M-SAA3 to test its ability to reduce intramammary infections with Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, and Escherichia coli, which are all common mastitis-producing pathogens. Recombinant production of M-SAA3 (followed by lipopolysaccharide removal to avoid lipopolysaccharide-nonspecific stimulation of the immune system) was successfully achieved. Mammary serum amyloid A3 stimulated the expression of IL-8 in a dose-dependent manner in primary mammary cultures. Although a direct killing effect on Staph. aureus by M-SAA3 was not detected, this acute phase protein was able to reduce Staph. aureus, Strep. uberis, and Strep. dysgalactiae infections by up to 50% and induced a reduction in E. coli counts of 67%. In general, the best concentration of caprine M-SAA3 for inhibiting infections was the lowest concentration tested (10 μg/mL), although higher concentrations (up to 160 μg/mL) increased its antimicrobial potential against some pathogens. 2025-10-22T11:18:56Z 2025-10-22T11:18:56Z 2020-02-11 info:eu-repo/semantics/article Parés, Sílvia, Francesc Fàbregas, Àlex Bach, Elena Garcia-Fruitós, Ana de Prado, and Anna Arís. 2020. "Short Communication: Recombinant Mammary Serum Amyloid A3 As A Potential Strategy For Preventing Intramammary Infections In Dairy Cows At Dryoff". Journal Of Dairy Science 103 (4): 3615-3621. doi:10.3168/jds.2019-17276. 0022-0302 http://hdl.handle.net/20.500.12327/876 https://doi.org/10.3168/jds.2019-17276 eng Journal of Dairy Science INIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2012-00028-C02-02/ES/Optimización del periodo de secado de la vaca mediante nanopartículas proteicas/ INIA/Programa Estatal de I+D+I orientada a los retos de la Sociedad/RTA2015-00064-C02/ES/Validación del uso de las proteínas M-SAA3 y MMP-9 en la mejora del secado de la vaca de leche y optimización de su dosis efectiva mediante su nanoestructuración/ http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 International American Dairy Science Association