2026-04-17T15:43:31Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/8752025-10-22T11:09:58Zcom_2072_4428com_2072_4427col_2072_487898
Potential of MMP-9 based nanoparticles at optimizing the cow dry period: pulling apart the effects of MMP-9 and nanoparticles
Gifre-Renom, L.
Carratalá, J. V.
Parés, S.
Sánchez-García, L.
Ferrer-Miralles, N.
Villaverde, A.
Bach, A.
Garcia-Fruitos, Elena
Alomar, Oscar
Producció Animal
Producció de Remugants
The cow dry period is a non-milking interval where the mammary gland involutes and regenerates
to guarantee an optimal milk production in the subsequent lactation. Important bottlenecks such
as the high risk of intramammary infections complicate the process. Antibiotics have been routinely
used as a preventive treatment but the concerns about potential antibiotic resistance open a new
scenario in which alternative strategies have to be developed. Matrix metalloproteinase-9 (MMP-9) is
an enzyme able to degrade the extracellular matrix, triggering the involution and immune function of
cow mammary gland. We have studied the infusion into the mammary gland of MMP-9 inclusion bodies
as protein-based nanoparticles, demonstrating that 1.2 mg of MMP-9 enhanced the involution and
immune function of the cow mammary gland. However, the comparison of the efects triggered by the
administration of an active and an inactive form of MMP-9 led to conclude that the response observed
in the bovine mammary gland was mainly due to the protein format but not to the biological activity of
the MMP-9 embedded in the inclusion body. This study provides relevant information on the future use
of protein inclusion bodies in cow mammary gland and the role of MMP-9 at dry-of.
2025-10-22T11:09:58Z
2025-10-22T11:09:58Z
2025-10-22T11:09:58Z
2020-07-09
info:eu-repo/semantics/article
Gifre-Renom, L., J. V. Carratalá, S. Parés, L. Sánchez-García, N. Ferrer-Miralles, A. Villaverde, A. Bach, Elena Garcia-Fruitós, and Anna Arís. 2020. "Potential Of MMP-9 Based Nanoparticles At Optimizing The Cow Dry Period: Pulling Apart The Effects Of MMP-9 And Nanoparticles". Scientific Reports 10 (1). doi:10.1038/s41598-020-67176-2.
2045-2322
http://hdl.handle.net/20.500.12327/875
https://doi.org/10.1038/s41598-020-67176-2
eng
Scientific Reports
INIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2012-00028-C02-01/ES/Optimización del periodo de secado de la vaca mediante nanopartículas proteicas/
INIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2012-00028-C02-02/ES/Optimización del periodo de secado de la vaca mediante nanopartículas proteicas/
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00064-C02-01/ES/Validación del uso de las proteínas M-SAA3 y MMP-9 en la mejora del secado de la vaca de leche y optimización de su dosis efectiva mediante su nanoestructuración/
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00064-C02-02/ES/Validación del uso de las proteínas M-SAA3 y MMP-9 en la mejora del secado de la vaca de leche y optimización de su dosis efectiva mediante su nanoestructuración/
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Attribution 4.0 International
Nature Research