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oai:recercat.cat:20.500.12327/792025-10-22T11:03:04Zcom_2072_4428com_2072_4427col_2072_487898

Role of AMPK signalling pathway during compensatory growth in pigs Ballester, Maria Amills, Marcel González-Rodríguez, Olga Cardoso, Tainã F. Pascual, Mariam González-Prendes, Rayner Panella-Riera, Núria Díaz, Isabel Tibau, Joan Quintanilla, Raquel Producció Animal Indústries Alimentàries Genètica i Millora Animal Qualitat i Tecnologia Alimentària Background: The molecular basis of compensatory growth in monogastric animals has not yet been fully explored. Herewith, in this study we aim to determine changes in the pig skeletal muscle transcriptome profile during compensatory growth following a feed restriction period. A RNA-Seq experiment was performed with a total of 24 females belonging to a Duroc commercial line. Half of the animals received either a restricted (RE) or ad libitum (AL) diet during the first fattening period (60–125 d of age). After that, all gilts were fed ad libitum for a further ~30 d until the age of ~155 d, when animals were slaughtered and samples of gluteus medius muscle were harvested to perform RNA-Seq analyses and intramuscular fat content determination. Results: During the period following food restriction, RE animals re-fed ad libitum displayed compensatory growth, showed better feed conversion rate and tended to deposit more subcutaneous fat than AL fed animals. Animals were slaughtered in the phase of accelerated growth, when RE animals had not completely compensated the performance of AL group, showing lower live and carcass weights. At intramuscular level, RE gilts showed a higher content of polyunsaturated fatty acids during the compensatory growth phase. The comparison of RE and AL expression profiles allowed the identification of 86 (ǀlog2Fold-Changeǀ > 1, padj < 0.05) differentially expressed (DE) genes. A functional categorization of these DE genes identified AMPK Signaling as the most significantly enriched canonical pathway. This kinase plays a key role in the maintenance of energy homeostasis as well as in the activation of autophagy. Among the DE genes identified as components of AMPK Signaling pathway, five out of six genes were downregulated in RE pigs. Conclusions: Animals re-fed after a restriction period exhibited a less oxidative metabolic profile and catabolic processes in muscle than animals fed ad libitum. The downregulation of autophagy observed in the skeletal muscle of pigs undergoing compensatory growth may constitute a mechanism to increase muscle mass thus ensuring an accelerated growth rate. These results reveal that the downregulation of AMPK Signaling plays an important role in compensatory growth in pigs. 2025-10-22T11:03:04Z 2025-10-22T11:03:04Z 2025-10-22T11:03:04Z 2018-09-17 info:eu-repo/semantics/article Ballester, M., Amills, M., González-Rodríguez, O., Cardoso, T., Pascual, M., & González-Prendes, R. et al. (2018). Role of AMPK signalling pathway during compensatory growth in pigs. BMC Genomics, 19(1). doi:10.1186/s12864-018-5071-5 1471-2164 http://hdl.handle.net/20.500.12327/79 https://doi.org/10.1186/s12864-018-5071-5 eng BMC Genomics MINECO/AGL2013/AGL2013-48742-C2-2-R/ES/FISIOLOGIA GENOMICA DEL DEPOSITO DE GRASA INTRAMUSCULAR EN PORCINO/ MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ / http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Attribution 4.0 International BioMed Central