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oai:recercat.cat:20.500.12327/7842025-10-22T11:31:22Zcom_2072_4428com_2072_4427col_2072_487898

Detection of Porcine Circovirus 3 in Wildlife Species in Spain Czyżewska-Dors, Ewelina Núñez, José I. Saporiti, Viviane Huerta, Eva Riutord, Carme Cabezón, Oscar Segalés, Joaquim Sibila, Marina Producció Animal Sanitat Animal 619 Porcine circovirus 3 (PCV-3) is the third member of the family Circoviridae, genus Circovirus, able to infect swine. A high prevalence of viral DNA has been recorded in wild boars. Recently, PCV-3 DNA was identified in Italian wild ruminants. Based on these previous results, this study assessed the frequency of PCV-3 DNA detection in free-ranging ruminants and Lagomorpha species in Spain. In addition, the genetic characterization of the PCV-3 PCR-positive samples was performed. A total of 801 serum samples, including red deer (Cervus elaphus, [CE]; n = 108), roe deer (Capreolus capreolus, [CC]; n = 87), Pyrenean chamois (Rupicapra pyrenaica, [RP]; n = 133), Iberian ibex (Capra pyrenaica, [CP]; n = 92), mouflon (Ovis aries, [OA]; n = 91), fallow deer (Dama dama, [DD]; n = 104), European rabbit (Oryctolagus cuniculus, [OC]; n = 101), and European hare (Lepus europaeus, [LE]; n = 85) from Catalonia (northeast Spain) were tested by conventional polymerase chain reaction (PCR) and, when positive, sequenced. Overall, PCV-3 DNA was found in three out of 801 analyzed sera (0.37%) corresponding to one red deer (1/108, 0.9%), one mouflon (1/91, 1.1%), and one fallow deer (1/104, 0.96%). None of the samples collected from Lagomorpha species resulted PCR positive. The partial genome sequences detected in positive samples displayed high identity with some PCV-3 sequences detected in wild boars and domestic pigs (99.7% and 100%, respectively). In conclusion, the present study indicated that free-ranging ruminant and Lagomorpha species are not relevant in the epidemiology of PCV-3 in Spain. info:eu-repo/semantics/publishedVersion 2020-05-01 info:eu-repo/semantics/article Czyżewska-Dors, Ewelina, José I. Núñez, Viviane Saporiti, Eva Huerta, Carme Riutord, Oscar Cabezón, Joaquim Segalés, and Marina Sibila. 2020. "Detection Of Porcine Circovirus 3 In Wildlife Species In Spain". Pathogens 9 (5): 341. MDPI AG. doi:10.3390/pathogens9050341. 2076-0817 http://hdl.handle.net/20.500.12327/784 https://doi.org/10.3390/pathogens9050341 eng Pathogens MINECO/Programa estatal de I+D+I orientada a los retos de la sociedad/E-RTA2017-00007-00-00/ES/Impacto de la infección por un nuevo circovirus porcino (PCV3) en España: desarrollo de herramientas diagnósticas, asociación con enfermedad y exploración de métodos de prevención y control/ Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess 6 MDPI