2026-04-18T01:11:28Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/7842025-10-22T11:31:22Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Czyżewska-Dors, Ewelina author Núñez, José I. author Saporiti, Viviane author Huerta, Eva author Riutord, Carme author Cabezón, Oscar author Segalés, Joaquim author Sibila, Marina author 2020-05-01 Porcine circovirus 3 (PCV-3) is the third member of the family Circoviridae, genus Circovirus, able to infect swine. A high prevalence of viral DNA has been recorded in wild boars. Recently, PCV-3 DNA was identified in Italian wild ruminants. Based on these previous results, this study assessed the frequency of PCV-3 DNA detection in free-ranging ruminants and Lagomorpha species in Spain. In addition, the genetic characterization of the PCV-3 PCR-positive samples was performed. A total of 801 serum samples, including red deer (Cervus elaphus, [CE]; n = 108), roe deer (Capreolus capreolus, [CC]; n = 87), Pyrenean chamois (Rupicapra pyrenaica, [RP]; n = 133), Iberian ibex (Capra pyrenaica, [CP]; n = 92), mouflon (Ovis aries, [OA]; n = 91), fallow deer (Dama dama, [DD]; n = 104), European rabbit (Oryctolagus cuniculus, [OC]; n = 101), and European hare (Lepus europaeus, [LE]; n = 85) from Catalonia (northeast Spain) were tested by conventional polymerase chain reaction (PCR) and, when positive, sequenced. Overall, PCV-3 DNA was found in three out of 801 analyzed sera (0.37%) corresponding to one red deer (1/108, 0.9%), one mouflon (1/91, 1.1%), and one fallow deer (1/104, 0.96%). None of the samples collected from Lagomorpha species resulted PCR positive. The partial genome sequences detected in positive samples displayed high identity with some PCV-3 sequences detected in wild boars and domestic pigs (99.7% and 100%, respectively). In conclusion, the present study indicated that free-ranging ruminant and Lagomorpha species are not relevant in the epidemiology of PCV-3 in Spain. Czyżewska-Dors, Ewelina, José I. Núñez, Viviane Saporiti, Eva Huerta, Carme Riutord, Oscar Cabezón, Joaquim Segalés, and Marina Sibila. 2020. "Detection Of Porcine Circovirus 3 In Wildlife Species In Spain". Pathogens 9 (5): 341. MDPI AG. doi:10.3390/pathogens9050341. 2076-0817 http://hdl.handle.net/20.500.12327/784 https://doi.org/10.3390/pathogens9050341 Detection of Porcine Circovirus 3 in Wildlife Species in Spain