2026-04-13T16:48:29Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/6792025-10-22T11:06:08Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Rossignoli, Araceli E.
author
Tudó, Angels
author
Bravo, Isabel
author
Díaz, Patricio A.
author
Diogène, Jorge
author
Riobó, Pilar
other
Producció Animal
group
Aigües Marines i Continentals
2025-10-22T11:06:08Z
2025-10-22T11:06:08Z
2020-02-21
Rossignoli, Araceli E., Angels Tudó, Isabel Bravo, Patricio A. Díaz, Jorge Diogène, and Pilar Riobó. 2020. "Toxicity Characterisation Of Gambierdiscus Species From The Canary Islands". Toxins 12 (2): 134. MDPI AG. doi:10.3390/toxins12020134.
2072-6651
http://hdl.handle.net/20.500.12327/679
https://doi.org/10.3390/toxins12020134
In the last decade, several outbreaks of ciguatera fish poisoning (CFP) have been reported in the Canary Islands (central northeast Atlantic Ocean), confirming ciguatera as an emerging alimentary risk in this region. Five Gambierdiscus species, G. australes, G. excentricus, G. silvae, G. carolinianus and G. caribaeus, have been detected in macrophytes from this area and are known to produce the ciguatoxins (CTXs) that cause CFP. A characterization of the toxicity of these species is the first step in identifying locations in the Canary Islands at risk of CFP. Therefore, in this study the toxicity of 63 strains of these five Gambierdiscus species were analysed using the erythrocyte lysis assay to evaluate their maitotoxin (MTX) content. In addition, 20 of the strains were also analysed in a neuroblastoma Neuro-2a (N2a) cytotoxicity assay to determine their CTX-like toxicity. The results allowed the different species to be grouped according to their ratios of CTX-like and MTX-like toxicity. MTX-like toxicity was especially high in G. excentricus and G. australes but much lower in the other species and lowest in G. silvae. CTX-like toxicity was highest in G. excentricus, which produced the toxin in amounts ranging between 128.2 ± 25.68 and 510.6 ± 134.2 fg CTX1B equivalents (eq) cell−1 (mean ± SD). In the other species, CTX concentrations were as follows: G. carolinianus (100.84 ± 18.05 fg CTX1B eq cell−1), G. australes (31.1 ± 0.56 to 107.16 ± 21.88 fg CTX1B eq cell−1), G. silvae (12.19 ± 0.62 to 76.79 ± 4.97 fg CTX1B eq cell−1) and G. caribaeus (<LOD to 90.37 ± 15.89 fg CTX1B eq cell−1). Unlike the similar CTX-like toxicity of G. australes and G. silvae strains from different locations, G. excentricus and G. caribaeus differed considerably according to the origin of the strain. These differences emphasise the importance of species identification to assess the regional risk of CFP.
eng
Attribution 4.0 International
Toxicity characterisation of Gambierdiscus species from the Canary Islands
info:eu-repo/semantics/article