2026-04-17T23:15:34Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/6542025-10-22T11:10:47Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Cortey, Martí author Díaz, Ivan author Vidal, Anna author Martín-Valls, Gerard author Franzo, Giovanni author Gómez de Nova, Pedro José author Darwich, Laila author Puente, Héctor author Carvajal, Ana author Martín, Marga author Mateu, Enric author 2019-12-05 BACKGROUND: Diarrhoea is a major cause of death in neonate pigs and most of the viruses that cause it are RNA viruses. Next Generation Sequencing (NGS) deeply characterize the genetic diversity among rapidly mutating virus populations at the interspecific as well as the intraspecific level. The diversity of RNA viruses present in faeces of neonatal piglets suffering from diarrhoea in 47 farms, plus 4 samples from non-diarrhoeic piglets has been evaluated by NGS. Samples were selected among the cases submitted to the Veterinary Diagnostic Laboratories of Infectious Diseases of the Universitat Autònoma de Barcelona (Barcelona, Spain) and Universidad de León (León, Spain). RESULTS: The analyses identified the presence of 12 virus species corresponding to 8 genera of RNA viruses. Most samples were co-infected by several viruses. Kobuvirus and Rotavirus were more commonly reported, with Sapovirus, Astrovirus 3, 4 and 5, Enterovirus G, Porcine epidemic diarrhoea virus, Pasivirus and Posavirus being less frequently detected. Most sequences showed a low identity with the sequences deposited in GenBank, allowing us to propose several new VP4 and VP7 genotypes for Rotavirus B and Rotavirus C. CONCLUSIONS: Among the cases analysed, Rotaviruses were the main aetiological agents of diarrhoea in neonate pigs. Besides, in a small number of cases Kobuvirus and Sapovirus may also have an aetiological role. Even most animals were co-infected in early life, the association with enteric disease among the other examined viruses was unclear. The NGS method applied successfully characterized the RNA virome present in faeces and detected a high level of unreported intraspecific diversity. Cortey, Martí, Ivan Díaz, Anna Vidal, Gerard Martín-Valls, Giovanni Franzo, Pedro José Gómez de Nova, and Laila Darwich et al. 2019. "High Levels Of Unreported Intraspecific Diversity Among RNA Viruses In Faeces Of Neonatal Piglets With Diarrhoea". BMC Veterinary Research 15 (1). Springer Science and Business Media LLC. doi:10.1186/s12917-019-2204-2. 1746-6148 http://hdl.handle.net/20.500.12327/654 https://doi.org/10.1186/s12917-019-2204-2 High levels of unreported intraspecific diversity among RNA viruses in faeces of neonatal piglets with diarrhoea