2026-04-17T19:10:32Zhttps://recercat.cat/oai/request
oai:recercat.cat:20.500.12327/6422025-10-22T11:01:46Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT author In-Cheol, Cho author Hee-Bok, Park author Jin Seop, Ahn author Sang-Hyun, Han author Jae-Bong, Lee author Hyun-Tae, Lim author Chae-Kyoung, Yoo author Eun-Ji, Jung author Dong-Hwan, Kim author Wu-Sheng, Sun author Ramayo-Caldas, Yuliaxis author Sang-Geum, Kim author Yong-Jun, Kang author Yoo-Kyung, Kim author Hyun-Sook, Shin author Pil-Nam, Seong author In-Sul, Hwang author Beom-Young, Park author Seongsoo, Hwang author Sung-Soo, Lee author Youn-Chul, Ryu author Jun-Heon, Lee author Moon-Suck, Ko author Kichoon, Lee author Andersson, Göran author Pérez-Enciso, Miguel author Jeong-Jeong, Lee other Producció Animal group Genètica i Millora Animal 2025-10-22T11:01:46Z 2025-10-22T11:01:46Z 2019-10-11 Cho, In-Cheol, Hee-Bok Park, Jin Seop Ahn, Sang-Hyun Han, Jae-Bong Lee, Hyun-Tae Lim, and Chae-Kyoung Yoo et al. 2019. "A Functional Regulatory Variant Of MYH3 Influences Muscle Fiber-Type Composition And Intramuscular Fat Content In Pigs". PLOS Genetics 15 (10): e1008279. Public Library of Science (PLoS). doi:10.1371/journal.pgen.1008279. 1553-7390 http://hdl.handle.net/20.500.12327/642 https://doi.org/10.1371/journal.pgen.1008279 Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5′-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs. eng Attribution 4.0 International A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs info:eu-repo/semantics/article