2026-04-17T05:12:24Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/522025-10-22T11:03:21Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Ruiz-Sánchez, J.
author
Guivernau, M.
author
Fernández, B.
author
Vila, J.
author
Viñas, M.
author
Riau, V.
author
Prenafeta-Boldú, F.X.
other
Producció Animal
group
Sostenibilitat en Biosistemes
2025-10-22T11:03:21Z
2025-10-22T11:03:21Z
2018-08-14
J. Ruiz-Sánchez, M. Guivernau, B. Fernández, J. Vila, M. Viñas, V. Riau, F.X. Prenafeta-Boldú, Functional biodiversity and plasticity of methanogenic biomass from a full-scale mesophilic anaerobic digester treating nitrogen-rich agricultural wastes, Science of The Total Environment, Volume 649, 2019, Pages 760-769
0048-9697
http://hdl.handle.net/20.500.12327/52
https://doi.org/10.1016/j.scitotenv.2018.08.165
The effect of ammonia on methanogenic biomass from a full-scale agricultural digester treating nitrogen-rich materials was characterized in batch activity assays subjected to increasing concentrations of total ammonia N. Acetotrophic and methanogenic profiles displayed prolonged lag phases and reduced specific activity rates at 6.0 gN-TAN L−1, though identical methane yields were ultimately reached. These results agreed with the expression levels of selected genes from bacteria and methanogenic archaea (qPCR of 16S rRNA and mrcA cDNA transcripts). Compound-specific isotope analysis of biogas indicated that ammonia exposure was associated to a transition in methanogenic activity from acetotrophy at 1.0 gN-TAN L−1 to intermediate and complete hydrogenotrophy at 3.5 and 6.0 gN-TAN L−1. Such pattern matched the results of 16S-Illumina sequencing of genes and transcripts in that predominant methanogens shifted, along with increasing ammonia, from the obligate acetotroph Methanosaeta to the hydrogenotrophic Methanoculleus and the poorly understood methylotrophic Methanomassiliicoccus. The underlying bacterial community structure remained rather stable but, at 6.0 gN-TAN L−1, the expression level increased considerably for a number of ribotypes that are related to potentially syntrophic genera (e.g. Clostridium, Bellilinea, Longilinea, and Bacteroides). The predominance of hydrogenotrophy at high ammonia levels clearly points to the occurrence of the syntrophic acetate oxidation (SAO), but known SAO bacteria were only found in very low numbers. The potential role of the identified bacterial and archaeal taxa with a view on SAO and on stability of the anaerobic digestion process under ammonia stress has been discussed.
eng
Attribution-NonCommercial-NoDerivatives 4.0 International
Functional biodiversity and plasticity of methanogenic biomass from a full-scale mesophilic anaerobic digester treating nitrogen-rich agricultural wastes
info:eu-repo/semantics/article