2026-04-17T20:40:40Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/5102025-10-22T11:25:07Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Toldrà, Anna
author
Alcaraz, Carles
author
Diogène, Jorge
author
O'Sullivan, Ciara K.
author
Campàs, Mònica
other
Producció Animal
group
Aigües Marines i Continentals
2025-10-22T11:25:07Z
2025-10-22T11:25:07Z
2019-06-28
Toldrà, Anna, Carles Alcaraz, Jorge Diogène, Ciara K. O'Sullivan, and Mònica Campàs. 2019. "Detection Of Ostreopsis Cf. Ovata In Environmental Samples Using An Electrochemical DNA-Based Biosensor". Science Of The Total Environment 689: 655-661. Elsevier BV. doi:10.1016/j.scitotenv.2019.06.448.
0048-9697
http://hdl.handle.net/20.500.12327/510
https://doi.org/10.1016/j.scitotenv.2019.06.448
Ostreopsis cf. ovata is a benthic microalga distributed in tropical and temperate regions worldwide which produces palytoxins (PlTXs). Herein, an electrochemical biosensor for the detection of this toxic microalga is described. The detection strategy involves isothermal recombinase polymerase amplification (RPA) of the target using tailed primers and a sandwich hybridisation assay on maleimide-coated magnetic beads immobilised on electrode arrays. The biosensor attained a limit of detection of 9 pg/μL of O. cf. ovata DNA (which corresponds to ~640 cells/L), with no interferences from two non-target Ostreopsis species (O. cf. siamensis and O. fattorussoi). The biosensor was applied to the analysis of planktonic and benthic environmental samples. Electrochemical O. cf. ovata DNA quantifications demonstrated an excellent correlation with other molecular methods (qPCR and colorimetric assays) and allowed the construction of a predictive regression model to estimate O. cf. ovata cell abundances. This new technology offer great potential to improve research, monitoring and management of O. cf. ovata and harmful algal blooms.
eng
Attribution-NonCommercial-NoDerivatives 4.0 International
Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor
info:eu-repo/semantics/article