2026-04-18T02:57:04Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/5102025-10-22T11:25:07Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Toldrà, Anna author Alcaraz, Carles author Diogène, Jorge author O'Sullivan, Ciara K. author Campàs, Mònica author 2019-06-28 Ostreopsis cf. ovata is a benthic microalga distributed in tropical and temperate regions worldwide which produces palytoxins (PlTXs). Herein, an electrochemical biosensor for the detection of this toxic microalga is described. The detection strategy involves isothermal recombinase polymerase amplification (RPA) of the target using tailed primers and a sandwich hybridisation assay on maleimide-coated magnetic beads immobilised on electrode arrays. The biosensor attained a limit of detection of 9 pg/μL of O. cf. ovata DNA (which corresponds to ~640 cells/L), with no interferences from two non-target Ostreopsis species (O. cf. siamensis and O. fattorussoi). The biosensor was applied to the analysis of planktonic and benthic environmental samples. Electrochemical O. cf. ovata DNA quantifications demonstrated an excellent correlation with other molecular methods (qPCR and colorimetric assays) and allowed the construction of a predictive regression model to estimate O. cf. ovata cell abundances. This new technology offer great potential to improve research, monitoring and management of O. cf. ovata and harmful algal blooms. Toldrà, Anna, Carles Alcaraz, Jorge Diogène, Ciara K. O'Sullivan, and Mònica Campàs. 2019. "Detection Of Ostreopsis Cf. Ovata In Environmental Samples Using An Electrochemical DNA-Based Biosensor". Science Of The Total Environment 689: 655-661. Elsevier BV. doi:10.1016/j.scitotenv.2019.06.448. 0048-9697 http://hdl.handle.net/20.500.12327/510 https://doi.org/10.1016/j.scitotenv.2019.06.448 Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor