2026-04-17T11:43:17Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/48082026-02-28T23:31:51Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Fernández Veiga, Leire
author
Fuertes, Miguel
author
Geijo, María V.
author
Elguezabal, Natalia
author
Serrano-Mestre, Jose L.
author
Vázquez-Iniesta, Lucía
author
Prados-Rosales, Rafael
author
Michelet, Lorraine
author
Boschiroli, Maria Laura
author
Pérez de Val, Bernat
author
Jones, Gareth J.
author
Juste, Ramón A.
author
Garrido, Joseba M.
author
Sevilla, Iker A.
other
Producció Animal
group
Sanitat Animal
2025-07-21
Fernández-Veiga, Leire, Miguel Fuertes, María V Geijo, Natalia Elguezabal, Jose L Serrano-Mestre, Lucía Vázquez-Iniesta, Rafael Prados-Rosales, Bernat Pérez de Val, et al. 2025. “Protection and Diagnostic Interference Induced by Heat-inactivated, Phage-inactivated and Live Vaccine Prototypes Against Animal Tuberculosis.” Frontiers in Veterinary Science 12 (July). https://doi.org/10.3389/fvets.2025.1620497.
2297-1769
http://hdl.handle.net/20.500.12327/4808
https://doi.org/10.3389/fvets.2025.1620497
Introduction: Vaccination emerges as a promising cost-eective tool to reduce
the impact and spread of animal tuberculosis, especially in regions where testand-slaughter eradication strategy is socioeconomically unfeasible or unfruitful
for dierent reasons, provided it is safe, e cacious and compatible with
diagnosis.
Methods: In this study, we preliminarily evaluated the diagnostic interference
(using guinea pigs) and the protective e cacy (using mice) of three heatinactivated, three phage-inactivated and one live attenuated vaccine prototypes
prepared from M. bovis, M. caprae, and M. microti.
Results and discussion: Phage-inactivation killed almost all (96.41–99.92%)
bacteria to be included in vaccines and filtering was used to remove the
remaining viable cells. All the assayed vaccines induced skin test reactions in
response to bovine tuberculin, but they were smaller in the phage-inactivated
vaccine groups. All the vaccines were diagnosis-compatible with defined skin
test antigens based on ESAT-6, CFP-10, and Rv3615c. In contrast with the
rest of prototypes, vaccination with heat- and phage-inactivated M. microti
did not prompt the production of detectable anti-MPB70+MPB83 antibodies.
Mean bacterial burden was lower in all vaccinated groups in comparison with
the control, being significantly reduced in the lungs of the heat-inactivated M.
microti and M. caprae and phage-inactivated M. caprae groups. Considering
both diagnostic interference and protection collectively, the heat-inactivated M.
microti vaccine showed the best performance. Further studies to evaluate these
vaccines and to improve phage-driven inactivation are warranted.
eng
Attribution 4.0 International
Protection and diagnostic interference induced by heat-inactivated, phage-inactivated and live vaccine prototypes against animal tuberculosis
info:eu-repo/semantics/article