2026-04-14T08:56:11Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/47322025-10-22T11:10:06Zcom_2072_4428com_2072_4427col_2072_487898

Comparison of Three Commercial ELISA Kits for Detection of Antibodies Against SARS-CoV-2 in Serum Samples from Different Animal Species Fernández-Bastit, Leira Marfil, Sílvia Pradenas, Edwards Blanco, Julià Vergara-Alert, Júlia Segalés, Joaquim Producció Animal Sanitat Animal 619 The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the coronavirus disease 19 (COVID-19) pandemic, significantly impacting global health, economies, and social stability. In February 2020, the first cases of SARS-CoV-2 infections in animals were documented, highlighting the potential risks posed by regular human–animal interactions in facilitating viral transmission. In consequence, it is essential to validate surveillance methods for SARS-CoV-2 in animals. In the present study, 101 sera from different animal species (36 cats, 41 dogs, 4 ferrets, 10 wild boar, 6 domestic goats, and 4 lions) were tested using three different ELISA kits to evaluate humoral responses against SARS-CoV-2. ELISA results were compared and correlated with a pseudovirus neutralization test (pVNT), considered as the reference assay. ELISA-1, targeting the receptor binding domain (RBD) neutralizing antibodies (nAbs) of SARS-CoV-2, exhibited the highest diagnostic performance, and proved to be a reliable tool for initial screenings in high-throughput animal studies. In contrast, ELISA-2 (also targeting RBD nAbs) and ELISA-3 (targeting nucleoprotein antibodies) demonstrated lower sensitivity for detecting seropositive animals. This research was partially funded by the BBVA Foundation as part of the project ‘Investigation on the potential role of pets as animal reservoirs for SARS-CoV-2’. The authors also acknowledge the crowdfunding initiative #Yomecorono, available online at https://www.yomecorono.com (accessed on 12 December 2024). IRTA (2021 SGR 00825 and 2021 SGR 01385) and IrsiCaixa (2021 SGR 00452) are supported by CERCA Programme/Generalitat de Catalunya. info:eu-repo/semantics/publishedVersion 2025-05-16 info:eu-repo/semantics/article Fernández-Bastit, Leira, Sílvia Marfil, Edwards Pradenas, Julià Blanco, Júlia Vergara-Alert, and Joaquim Segalés. 2025. “Comparison of Three Commercial ELISA Kits for Detection of Antibodies Against SARS-CoV-2 in Serum Samples From Different Animal Species.” Viruses 17 (5): 716. https://doi.org/10.3390/v17050716. 1999-4915 http://hdl.handle.net/20.500.12327/4732 https://doi.org/10.3390/v17050716 eng Viruses Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess 16 MDPI