2026-04-14T02:31:26Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/47322025-10-22T11:10:06Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Fernández-Bastit, Leira
author
Marfil, Sílvia
author
Pradenas, Edwards
author
Blanco, Julià
author
Vergara-Alert, Júlia
author
Segalés, Joaquim
other
Producció Animal
group
Sanitat Animal
2025-10-22T11:10:06Z
2025-10-22T11:10:06Z
2025-05-16
Fernández-Bastit, Leira, Sílvia Marfil, Edwards Pradenas, Julià Blanco, Júlia Vergara-Alert, and Joaquim Segalés. 2025. “Comparison of Three Commercial ELISA Kits for Detection of Antibodies Against SARS-CoV-2 in Serum Samples From Different Animal Species.” Viruses 17 (5): 716. https://doi.org/10.3390/v17050716.
1999-4915
http://hdl.handle.net/20.500.12327/4732
https://doi.org/10.3390/v17050716
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the coronavirus disease 19 (COVID-19) pandemic, significantly impacting global health, economies, and social stability. In February 2020, the first cases of SARS-CoV-2 infections in animals were documented, highlighting the potential risks posed by regular human–animal interactions in facilitating viral transmission. In consequence, it is essential to validate surveillance methods for SARS-CoV-2 in animals. In the present study, 101 sera from different animal species (36 cats, 41 dogs, 4 ferrets, 10 wild boar, 6 domestic goats, and 4 lions) were tested using three different ELISA kits to evaluate humoral responses against SARS-CoV-2. ELISA results were compared and correlated with a pseudovirus neutralization test (pVNT), considered as the reference assay. ELISA-1, targeting the receptor binding domain (RBD) neutralizing antibodies (nAbs) of SARS-CoV-2, exhibited the highest diagnostic performance, and proved to be a reliable tool for initial screenings in high-throughput animal studies. In contrast, ELISA-2 (also targeting RBD nAbs) and ELISA-3 (targeting nucleoprotein antibodies) demonstrated lower sensitivity for detecting seropositive animals.
eng
Attribution 4.0 International
Comparison of Three Commercial ELISA Kits for Detection of Antibodies Against SARS-CoV-2 in Serum Samples from Different Animal Species
info:eu-repo/semantics/article