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oai:recercat.cat:20.500.12327/47322025-10-22T11:10:06Zcom_2072_4428com_2072_4427col_2072_487898

2025-10-22T11:10:06Z urn:hdl:20.500.12327/4732 Comparison of Three Commercial ELISA Kits for Detection of Antibodies Against SARS-CoV-2 in Serum Samples from Different Animal Species Fernández-Bastit, Leira Marfil, Sílvia Pradenas, Edwards Blanco, Julià Vergara-Alert, Júlia Segalés, Joaquim Producció Animal Sanitat Animal The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the coronavirus disease 19 (COVID-19) pandemic, significantly impacting global health, economies, and social stability. In February 2020, the first cases of SARS-CoV-2 infections in animals were documented, highlighting the potential risks posed by regular human–animal interactions in facilitating viral transmission. In consequence, it is essential to validate surveillance methods for SARS-CoV-2 in animals. In the present study, 101 sera from different animal species (36 cats, 41 dogs, 4 ferrets, 10 wild boar, 6 domestic goats, and 4 lions) were tested using three different ELISA kits to evaluate humoral responses against SARS-CoV-2. ELISA results were compared and correlated with a pseudovirus neutralization test (pVNT), considered as the reference assay. ELISA-1, targeting the receptor binding domain (RBD) neutralizing antibodies (nAbs) of SARS-CoV-2, exhibited the highest diagnostic performance, and proved to be a reliable tool for initial screenings in high-throughput animal studies. In contrast, ELISA-2 (also targeting RBD nAbs) and ELISA-3 (targeting nucleoprotein antibodies) demonstrated lower sensitivity for detecting seropositive animals. 2025-10-22T11:10:06Z 2025-10-22T11:10:06Z 2025-05-16 info:eu-repo/semantics/article Fernández-Bastit, Leira, Sílvia Marfil, Edwards Pradenas, Julià Blanco, Júlia Vergara-Alert, and Joaquim Segalés. 2025. “Comparison of Three Commercial ELISA Kits for Detection of Antibodies Against SARS-CoV-2 in Serum Samples From Different Animal Species.” Viruses 17 (5): 716. https://doi.org/10.3390/v17050716. 1999-4915 http://hdl.handle.net/20.500.12327/4732 https://doi.org/10.3390/v17050716 eng Viruses http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Attribution 4.0 International MDPI