2026-04-14T08:50:29Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/46842025-10-22T11:29:56Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Angen, Øystein author Karstensen, Kasper Thystrup author Vilaró, Anna author Cavaco, Lina Maria author Langford, Paul R author Sauce, Lorenzo Jose Fraile author Migura, Lourdes author Salomonsen, Charlotte Mark author Li, Yanwen author Bossé, Janine T author 2025-07-15 Serovar detector is a new bioinformatic tool for determining the serovar of Actinobacillus pleuropneumoniae using whole genome sequencing. The composition of cps genes of isolates is compared to those of the serovar reference strains, and the outcome is determined both by the number of common genes and the similarities between the homologous genes. The validation of the bioinformatic tool utilized a broad collection of 732 isolates, including representatives from all described serovars. The isolates included had been characterized by conventional serotyping, PCR tests or different bioinformatic tools. The collection also includes isolates that have been difficult to allocate to a serovar using serology to test the performance of the Serovar detector when potential new varieties or combinations of cps genes are present. Out of the 732 isolates included in the investigation, only 36 isolates (4.9%) could not be allocated to the 19 recognized serovars. The validation showed that the Serovar detector is a robust method for determining the serovar of an isolate and a valuable tool for further characterization of the genetic heterogeneity both within serovars and within the A. pleuropneumoniae species. Angen, Øystein, Kasper Thystrup Karstensen, Anna Vilaró, Lina Maria Cavaco, Paul R. Langford, Lorenzo Jose Fraile Sauce, Lourdes Migura-Garcia, Charlotte Mark Salomonsen, Yanwen Li, and Janine T. Bossé. 2025. “Serotyping of Actinobacillus Pleuropneumoniae Based on Whole Genome Sequencing: Validation of a Bioinformatic Tool.” Microbial Genomics 11 (7). https://doi.org/10.1099/mgen.0.001434. 2057-5858 http://hdl.handle.net/20.500.12327/4684 https://doi.org/10.1099/mgen.0.001434 Serotyping of Actinobacillus pleuropneumoniae based on whole genome sequencing: validation of a bioinformatic tool