2026-04-18T00:32:30Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/3872025-10-22T11:34:08Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Cardoso, Tainã Figueiredo
author
Quintanilla, Raquel
author
Castelló, Anna
author
González-Prendes, Rayner
author
Amills, Marcel
author
Cánovas, Ángela
other
Producció Animal
group
Genètica i Millora Animal
2025-10-22T11:34:07Z
2025-10-22T11:34:07Z
2018-02-14
Cardoso, Tainã Figueiredo, Raquel Quintanilla, Anna Castelló, Rayner González-Prendes, Marcel Amills, and Ángela Cánovas. 2018. "Differential Expression Of Mrna Isoforms In The Skeletal Muscle Of Pigs With Distinct Growth And Fatness Profiles". BMC Genomics 19 (1). Springer Nature. doi:10.1186/s12864-018-4515-2.
1471-2164
http://hdl.handle.net/20.500.12327/387
https://doi.org/10.1186/s12864-018-4515-2
Background: The identification of genes differentially expressed in the skeletal muscle of pigs displaying distinct
growth and fatness profiles might contribute to identify the genetic factors that influence the phenotypic variation
of such traits. So far, the majority of porcine transcriptomic studies have investigated differences in gene expression
at a global scale rather than at the mRNA isoform level. In the current work, we have investigated the differential
expression of mRNA isoforms in the gluteus medius (GM) muscle of 52 Duroc HIGH (increased backfat thickness,
intramuscular fat and saturated and monounsaturated fatty acids contents) and LOW pigs (opposite phenotype,
with an increased polyunsaturated fatty acids content).
Results: Our analysis revealed that 10.9% of genes expressed in the GM muscle generate alternative mRNA
isoforms, with an average of 2.9 transcripts per gene. By using two different pipelines, one based on the CLC
Genomics Workbench and another one on the STAR, RSEM and DESeq2 softwares, we have identified 10
mRNA isoforms that both pipelines categorize as differentially expressed in HIGH vs LOW pigs (P-value < 0.01
and ±0.6 log2fold-change). Only five mRNA isoforms, produced by the ITGA5, SEMA4D, LITAF, TIMP1 and ANXA2
genes, remain significant after correction for multiple testing (q-value < 0.05 and ±0.6 log2fold-change), being
upregulated in HIGH pigs.
Conclusions: The increased levels of specific ITGA5, LITAF, TIMP1 and ANXA2 mRNA isoforms in HIGH pigs is
consistent with reports indicating that the overexpression of these four genes is associated with obesity and
metabolic disorders in humans. A broader knowledge about the functional attributes of these mRNA variants
would be fundamental to elucidate the consequences of transcript diversity on the determinism of porcine
phenotypes of economic interest.
Keywords: Alternative splicing, mRNA isoform, Swine, Differential expression
eng
Attribution 4.0 International
Differential expression of mRNA isoforms in the skeletal muscle of pigs with distinct growth and fatness profiles
info:eu-repo/semantics/article