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oai:recercat.cat:20.500.12327/36722025-10-22T11:17:21Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Gonzalez-Ibeas, Daniel author Blanca, José author Roig, Cristina author González-To, Mireia author Picó, Belén author Truniger, Verónica author Gómez, Pedro author Deleu, Wim author Caño- Delgado, Ana I. author Arús, Pere author Nuez, Fernando author Garcia-Mas, Jordi author Puigdomènech, Pere author Aranda, Miguel A author 2007-09-03 Background: Melon (Cucumis melo L.) is one of the most important fleshy fruits for fresh consumption. Despite this, few genomic resources exist for this species. To facilitate the discovery of genes involved in essential traits, such as fruit development, fruit maturation and disease resistance, and to speed up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ESTs) from eight normalized cDNA libraries from different tissues in different physiological conditions. Results: We determined over 30,000 ESTs that were clustered into 16,637 non-redundant sequences or unigenes, comprising 6,023 tentative consensus sequences (contigs) and 10,614 unclustered sequences (singletons). Many potential molecular markers were identified in the melon dataset: 1,052 potential simple sequence repeats (SSRs) and 356 single nucleotide polymorphisms (SNPs) were found. Sixty-nine percent of the melon unigenes showed a significant similarity with proteins in databases. Functional classification of the unigenes was carried out following the Gene Ontology scheme. In total, 9,402 unigenes were mapped to one or more ontology. Remarkably, the distributions of melon and Arabidopsis unigenes followed similar tendencies, suggesting that the melon dataset is representative of the whole melon transcriptome. Bioinformatic analyses primarily focused on potential precursors of melon micro RNAs (miRNAs) in the melon dataset, but many other genes potentially controlling disease resistance and fruit quality traits were also identified. Patterns of transcript accumulation were characterised by Real-Time-qPCR for 20 of these genes. Conclusion: The collection of ESTs characterised here represents a substantial increase on the genetic information available for melon. A database (MELOGEN) which contains all EST sequences, contig images and several tools for analysis and data mining has been created. This set of sequences constitutes also the basis for an oligo-based microarray for melon that is being used in experiments to further analyse the melon transcriptome. Gonzalez-Ibeas, Daniel, José Blanca, Cristina Roig, Mireia González-To, Belén Picó, Verónica Truniger, Pedro Gómez, et al. 2007. “MELOGEN: An EST Database for Melon Functional Genomics.” BMC Genomics 8 (1): 306. doi: 10.1186/1471-2164-8-306 1471-2164 http://hdl.handle.net/20.500.12327/3672 https://doi.org/10.1186/1471-2164-8-306 MELOGEN: an EST database for melon functional genomics