2026-04-14T03:34:47Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/35992025-10-22T11:15:56Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Vasselon, Valentin author Rivera, Sinziana F. author Ács, Éva author Baja Almeida, Salomé author Andree, Karl author Apothéloz-Perret-Gentil, Laure author Bailet, Bonnie author Baričević, Ana author Beentjes, Kevin K. author Bettig, Juliane author Bouchez, Agnès author Capeli, Camilla author Chardon, Cécile author Duleba, Mónika author Elersek, Tina author Genthon, Clémence author Jablonska, Maša author Jacas, Louis author Kahlert, Maria author Kelly, Martyn G. author Macher, Jan-Niklas author Mauri, Federica author Moletta-Denat, Marina author Mortágua, Andreia author Pawlowski, Jan author Pérez Burillo, Javier author Pfannkuchen, Martin author Pilgrim, Erik author Pissaridou, Panayiota author Rimet, Frédéric author Stanic, Karmen author Tapolczai, Kálmán author Theroux, Susanna author Trobajo, Rosa author Van der Hoorn, Berry author Vasquez, Marlen I. author Vidal, Marie author Wanless, David author Warren, Jonathan author Zimmermann, Jonas author Paix, Benoît author 2025-01-10 DNA metabarcoding of benthic diatoms has been successfully applied for biomonitoring at the national scale and can now be considered technically ready for routine application. However, protocols and methods still vary between and within countries, limiting their transferability and the comparability of results. In order to overcome this, routine use of DNA metabarcoding for diatom biomonitoring requires knowledge of the sources of variability introduced by the different steps of the procedure. Here, we examine how elements of routine procedures contribute to variability between European laboratories. A set of four experiments were performed focusing on DNA extraction and PCR amplification steps to evaluate their reproducibility between different laboratories and the variability introduced by different protocols currently applied by the scientific community. Under the guidance of a reference laboratory, 17 participants from 14 countries performed DNA extraction and PCR amplification in parallel, using the same fixed protocol and their own choice of protocol. Experiments were performed by each participant on a set of standardised DNA and biofilm samples (river, lake and mock community) to investigate potential systematic and random errors. Our results revealed the successful transferability of a protocol amongst labs and a highly similar and consistent ecological assessment outcome obtained regardless of the protocols used by each participant. We propose an “all for one but prove them all” strategy, suggesting that distinct protocols can be used within the scientific community, as long as their consistency is be proven by following minimum standard requirements. Vasselon, Valentin, Sinziana F. Rivera, Éva Ács, Salomé Baja Almeida, Karl B. Andree, Laure Apothéloz-Perret-Gentil, Bonnie Bailet, et al. 2025. “Proficiency Testing and Cross-laboratory Method Comparison to Support Standardisation of Diatom DNA Metabarcoding for Freshwater Biomonitoring.” Metabarcoding and Metagenomics 9. https://doi.org/10.3897/mbmg.9.133264. 2534-9708 http://hdl.handle.net/20.500.12327/3599 https://doi.org/10.3897/mbmg.9.133264 Proficiency testing and cross-laboratory method comparison to support standardisation of diatom DNA metabarcoding for freshwater biomonitoring