2026-04-17T21:30:19Zhttps://recercat.cat/oai/request
oai:recercat.cat:20.500.12327/33872025-10-22T11:33:02Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT author Weber, Klaus author Mayoral, Francisco José author Vallejo, Carla author Sánchez, Raúl author Hartelust, Roberto author Mendoza, Paula author PÉREZ DE VAL, BERNAT author Savé, Jordi author Okazaki, Yoshimasa author Ortega, Paula author Rocamora, Laura author Sandoval, Albert author Vallejo, Raquel author de Miguel, Ricardo author Kegler, Kristel other Producció Animal group Sanitat Animal 2025-10-22T11:33:02Z 2025-10-22T11:33:02Z 2024-07-01 Weber, Klaus, Francisco José Mayoral, Carla Vallejo, Raúl Sánchez, Roberto Hartelust, Paula Mendoza, Val, et al. “Natural Outbreak of Mycobacterium Caprae Infection in Imported Laboratory Cynomolgus Macaques (Macaca Fascicularis): Diagnostic Pitfalls and Management of Safety Precautions.” Journal of Toxicologic Pathology 37 (4): 197–206. https://doi.org/10.1293/tox.2024-0048. 0914-9198 http://hdl.handle.net/20.500.12327/3387 https://doi.org/10.1293/tox.2024-0048 Tuberculosis (TB) is a major health threat for humans and for non-human primates used for toxicology or research purposes. Emerging mycobacterial species represent a major challenge for diagnosis and surveillance programs. Here, we report a natural outbreak of Mycobacterium caprae in imported cynomolgus macaques (Macaca fascicularis) that occurred at AnaPath Research S.A.U. (APR). The macaques underwent repeated negative intradermal tuberculin tests (IDT) before importation and at the European quarantine station. Exhaustive TB screening was started at APR after confirmation of one positive case at another facility. The animal in question belonged to the same colony received at APR. Diagnostic approaches included clinical examination, PCR, culture, spoligotyping, IDT testing, interferon-γ release assay (IGRA), and thoracoabdominal ultrasound (US). Three regulatory toxicity studies and stock animals were affected. The macaques lacked clinical signs, except for one showing a fistulizing nodule in the right inguinal area, which tested positive for the Mycobacterium tuberculosis complex by PCR. All animals were necropsied and 10 macaques (n=114) showed gross and histologic findings compatible with TB confirmed by PCR and culture. M. caprae was identified as the etiological agent by Direct Variable Repeat spacer oligonucleotide typing (DVR spoligotyping). The infection was traced to Asia via the SB1622 spoligotype involved, confirming that the animals were infected prior to their import into Europe. Tuberculin skin test (TST), IGRA, and US were only sensitive in detecting advanced cases of M. caprae infection. One staff member showed a positive TST reaction, which was handled in accordance with the Spanish government’s health regulations. All the sanitary measures implemented were effective in eradicating the disease. eng Attribution-NonCommercial-NoDerivatives 4.0 International Natural outbreak of Mycobacterium caprae infection in imported laboratory cynomolgus macaques (Macaca fascicularis): diagnostic pitfalls and management of safety precautions info:eu-repo/semantics/article