2026-04-18T04:56:18Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/33512025-10-22T11:08:35Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Casanovas Castro, Maria
author
Claveria, Elisabet
author
Dolcet-Sanjuan, Ramon
other
Producció Vegetal
group
Fructicultura
2025-10-22T11:08:35Z
2025-10-22T11:08:35Z
2024-10-22
Casanovas, Maria, Elisabet Claveria, and Ramon Dolcet-Sanjuan. 2024. “Development of a Feasible and Efficient In Vitro Rescue Protocol for Immature Prunus Spp. Embryos.” Plants, 13(21), 2953. https://doi.org/10.3390/plants13212953
2223-7747
http://hdl.handle.net/20.500.12327/3351
https://doi.org/10.3390/plants13212953
The major factors affecting the in vitro immature embryo rescue efficiencies from Prunus
persica or P. armeniaca accessions have been identified, along with improving the feasibility. Variations
in the woody plant medium (WPM) were used depending on the embryo size. Embryos less than
5 mm long were cultured in WPM supplemented with 1 µM BAP and 1 µM GA3, while embryos
bigger than 5 mm long were cultured in hormone-free medium, with or without vermiculite. The
environmental in vitro culture conditions consisted of three phases: a (I) stratification at 4 ◦C during
a 3- to 5-month-long period in the dark, followed by (II) growth of germinated embryos at 14 ◦C
for a 4-week-long period, with 12 h light a day, which favors plantlet development, and finally, (III)
growth at 24 ◦C, with 16 h light a day, until the plantlets were acclimatized in the greenhouse. The
germination of smaller embryos, at the end of phase I, ranged from 82.2% to 22.1% for apricots and
flat peaches, respectively, whereas for bigger embryos, the germination varied from 97.3% to 53.2% for
the same species. The embryo germination for peaches and nectarines ranged from 40.1% to 30.3% for
smaller embryos, and from 91.9% to 63.0% for bigger embryos. Endo- and epiphytic contamination,
affecting from 7.4% to 52.9% of cultured embryos, depending on the fruit type and conservation
conditions, and the capacity to acclimate to soil conditions, ranging from 50.4% to 93.2%, were the
two most important factors influencing the protocol’s efficiency and feasibility. Considering the
overall efficiencies, expressed as hardened plants transferred to field plots over clean uncontaminated
embryo, the values ranged from 55.8% for nectarines, 54.0% for peaches, 45.6% for apricots, and
23.3% for flat fruits. The addition of vermiculite to the culture medium significantly improved the
plantlet development, avoiding subculture to fresh medium when an extension of phase III was
required before acclimatization. Compared to laboratory glassware, the use of food glass containers
with air-permeable sealing film, along with vermiculite-containing medium, significantly reduced
the costs when handling the large number of embryos required for breeding programs.
eng
Attribution 4.0 International
Development of a Feasible and Efficient In Vitro Rescue Protocol for Immature Prunus spp. Embryos
info:eu-repo/semantics/article