2026-04-17T05:21:56Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/32682025-10-22T11:26:16Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Cabrera, Antonio
author
Kozik, Alex
author
Howad, Werner
author
Arús, Pere
author
Iezzoni, Amy F
author
van der Knaap, Esther
other
Producció Vegetal
group
Genòmica i Biotecnologia
2025-10-22T11:26:16Z
2025-10-22T11:26:16Z
2009-11-29
Cabrera, Antonio, Alex Kozik, Werner Howad, Pere Arus, Amy F Iezzoni, and Esther Van Der Knaap. 2009. “Development and Bin Mapping of a Rosaceae Conserved Ortholog Set (COS) of Markers.” BMC Genomics 10 (1): 562. doi: 10.1186/1471-2164-10-562
1471-2164
http://hdl.handle.net/20.500.12327/3268
https://doi.org/10.1186/1471-2164-10-562
Background: Detailed comparative genome analyses within the economically important Rosaceae
family have not been conducted. This is largely due to the lack of conserved gene-based molecular
markers that are transferable among the important crop genera within the family [e.g. Malus
(apple), Fragaria (strawberry), and Prunus (peach, cherry, apricot and almond)]. The lack of
molecular markers and comparative whole genome sequence analysis for this family severely
hampers crop improvement efforts as well as QTL confirmation and validation studies.
Results: We identified a set of 3,818 rosaceaous unigenes comprised of two or more ESTs that
correspond to single copy Arabidopsis genes. From this Rosaceae Conserved Orthologous Set
(RosCOS), 1039 were selected from which 857 were used for the development of intron-flanking
primers and allele amplification. This led to successful amplification and subsequent mapping of 613
RosCOS onto the Prunus TxE reference map resulting in a genome-wide coverage of 0.67 to 1.06
gene-based markers per cM per linkage group. Furthermore, the RosCOS primers showed
amplification success rates from 23 to 100% across the family indicating that a substantial part of
the RosCOS primers can be directly employed in other less studied rosaceaous crops.
Comparisons of the genetic map positions of the RosCOS with the physical locations of the
orthologs in the Populus trichocarpa genome identified regions of colinearity between the genomes
of Prunus-Rosaceae and Populus-Salicaceae.
Conclusion: Conserved orthologous genes are extremely useful for the analysis of genome
evolution among closely and distantly related species. The results presented in this study
demonstrate the considerable potential of the mapped Prunus RosCOS for genome-wide marker
employment and comparative whole genome studies within the Rosaceae family. Moreover, these
markers will also function as useful anchor points for the genome sequencing efforts currently
ongoing in this family as well as for comparative QTL analyses.
eng
Attribution 4.0 International
Development and bin mapping of a Rosaceae Conserved Ortholog Set (COS) of markers
info:eu-repo/semantics/article