2026-04-17T19:28:15Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/31042025-10-22T11:04:21Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
González, Víctor M
author
Garcia-Mas, Jordi
author
Arús, Pere
author
Puigdomènech, Pere
other
Producció Vegetal
group
Genòmica i Biotecnologia
2025-10-22T11:04:21Z
2025-10-22T11:04:21Z
2010-05-28
González, Víctor M, Jordi Garcia-Mas, Pere Arús, and Pere Puigdomènech. 2010. “Generation of a BAC-based Physical Map of the Melon Genome.” BMC Genomics 11 (1): 339. doi: 10.1186/1471-2164-11-339
1471-2164
http://hdl.handle.net/20.500.12327/3104
https://doi.org/10.1186/1471-2164-11-339
Background: Cucumis melo (melon) belongs to the Cucurbitaceae family, whose economic importance among
horticulture crops is second only to Solanaceae. Melon has high intra-specific genetic variation, morphologic diversity
and a small genome size (450 Mb), which make this species suitable for a great variety of molecular and genetic studies
that can lead to the development of tools for breeding varieties of the species. A number of genetic and genomic
resources have already been developed, such as several genetic maps and BAC genomic libraries. These tools are
essential for the construction of a physical map, a valuable resource for map-based cloning, comparative genomics and
assembly of whole genome sequencing data. However, no physical map of any Cucurbitaceae has yet been
developed. A project has recently been started to sequence the complete melon genome following a whole-genome
shotgun strategy, which makes use of massive sequencing data. A BAC-based melon physical map will be a useful tool
to help assemble and refine the draft genome data that is being produced.
Results: A melon physical map was constructed using a 5.7 × BAC library and a genetic map previously developed in
our laboratories. High-information-content fingerprinting (HICF) was carried out on 23,040 BAC clones, digesting with
five restriction enzymes and SNaPshot labeling, followed by contig assembly with FPC software. The physical map has
1,355 contigs and 441 singletons, with an estimated physical length of 407 Mb (0.9 × coverage of the genome) and the
longest contig being 3.2 Mb. The anchoring of 845 BAC clones to 178 genetic markers (100 RFLPs, 76 SNPs and 2 SSRs)
also allowed the genetic positioning of 183 physical map contigs/singletons, representing 55 Mb (12%) of the melon
genome, to individual chromosomal loci. The melon FPC database is available for download at http://
melonomics.upv.es/static/files/public/physical_map/.
Conclusions: Here we report the construction of the first physical map of a Cucurbitaceae species described so far. The
physical map was integrated with the genetic map so that a number of physical contigs, representing 12% of the
melon genome, could be anchored to known genetic positions. The data presented is already helping to improve the
quality of the melon genomic sequence available as a result of a project currently being carried out in Spain, adopting
a whole genome shotgun approach based on 454 sequencing data.
eng
Attribution 4.0 International
Generation of a BAC-based physical map of the melon genome
info:eu-repo/semantics/article