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oai:recercat.cat:20.500.12327/31022025-10-22T11:33:21Zcom_2072_4428com_2072_4427col_2072_487898

Virulence-associated trimeric autotransporters of Haemophilus parasuis are antigenic proteins expressed in vivo Olvera, Alex Pina-Pedrero, Sonia Perez-Simo, Marta Oliveira, Simone Bensaid, Albert Producció Animal Sanitat Animal Glässer’s disease is a re-emerging swine disease characterized by a severe septicaemia. Vaccination has been widely used to control the disease, although there is a lack of extended crossprotection. Trimeric autotransporters, a family of surface exposed proteins implicated in host-pathogen interactions, are good vaccine candidates. Members of this family have been described in Haemophilus parasuis and designated as virulence-associated trimeric autotransporters (VtaA). In this work, we produced 15 recombinant VtaA passenger domains and looked for the presence of antibodies directed against them in immune sera by immunoblotting. After infection with a subclinical dose of H. parasuis Nagasaki, an IgG mediated antibody response against 6 (VtaA1, 5, 6, 8, 9 and 10) of the 13 VtaA of the Nagasaki strain was detected, indicating that they are expressed in vivo. IgA production against VtaA was detected in only one animal. VtaA were more likely to be late antigens when compared to early (Omp P5 and Omp P6) and late (YaeT) defined antigens. Antibody cross-reaction with two orthologs of Nagasaki’s VtaA5 and 6, VtaA15 and 16 of strain HP1319, was also detected. No antibodies against VtaA were detected in the sera of animals immunized with a bacterin of the Nagasaki strain, suggesting poor expression in the in vitro conditions used. Taken together, these results indicate that VtaA are good candidate immunogens that could be used to improve H. parasuis vaccines. However, their capacity to confer protective immunity needs to be further studied. 2025-10-22T11:33:21Z 2025-10-22T11:33:21Z 2025-10-22T11:33:21Z 2009-12-10 info:eu-repo/semantics/article Olvera, Alex, Sonia Pina, Marta Pérez-Simó, Simone Oliveira, and Albert Bensaid. 2009. “Virulence-associated Trimeric Autotransporters ofHaemophilus Parasuisare Antigenic Proteins Expressed in Vivo.” Veterinary Research 41 (3): 26. doi: 10.1051/vetres/2009074 0928-4249 http://hdl.handle.net/20.500.12327/3102 https://doi.org/10.1051/vetres/2009074 eng Veterinary Research MEC/ /AGL2007-60432/ES/Caracterizacion funcional e inmunologica de las proteinas de superficie y proteinas secretadas de cepas virulentas de Haemophilus parasuis/GAN http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial 4.0 International EDP Sciences