2026-04-14T08:32:48Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/30572025-10-22T11:26:22Zcom_2072_4428com_2072_4427col_2072_487898

The ethylene-responsive transcription factor ERF024 is a novel regulator of climacteric fruit ripening in melon Santo Domingo, Miguel Orduña, Luis Navarro, David Mayobre, Carlos Santiago, Antonio Valverde, Laura Alexiou, Konstantinos Tomás Matus, José Pujol, Marta Garcia-Mas, Jordi Producció Vegetal Genòmica i Biotecnologia Fruit ripening is an essential developmental stage in Angiosperms triggered by hormonal signals such as ethylene, a major player in climacteric ripening. Melon is a unique crop showing both climacteric and non-climacteric cultivars, offering an ideal model for dissecting the genetic mechanisms underpinning this process. The major quantitative trait locus ETHQV8.1 was previously identified as a key regulator of melon fruit ripening. Here, we narrowed down ETHQV8.1 to a precise genomic region containing a single gene, the transcription factor CmERF024. Functional validation using CRISPR/Cas9 knock-out plants unequivocally identified CmERF024 as the causal gene governing ETHQV8.1. The erf024 mutants exhibited suppression of ethylene production, leading to a significant delay and attenuation of fruit ripening. Integrative multi-omic analyses encompassing RNA-seq, DAP-seq, and DNase-seq revealed the association of CmERF024 with chromatin accessibility and gene expression dynamics throughout fruit ripening. Our data suggest CmERF024 as a novel regulator of climacteric fruit ripening in melon. 2025-10-22T11:26:22Z 2025-10-22T11:26:22Z 2025-10-22T11:26:22Z 2024-06-20 info:eu-repo/semantics/article Santo Domingo, Miguel, Luis Orduña, David Navarro, Carlos Mayobre, Antonio Santiago, Laura Valverde, Konstantinos G. Alexiou, José Tomás Matus, Marta Pujol, and Jordi Garcia‐Mas. 2024. The Plant Journal, June. doi:10.1111/tpj.16889. 0960-7412 http://hdl.handle.net/20.500.12327/3057 https://doi.org/10.1111/tpj.16889 eng The Plant Journal MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-097665-B-C21/ES/APROXIMACIONES DE VARIABILIDAD NATURAL Y EDICIÓN GENÉTICA PARA EL ESTUDIO DE LA CALIDAD DE FRUTO Y LA RESISTENCIA A ENFERMEDADES EN MELÓN/ MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2021-125998OB-C21/ES/Natural variability and gene editing to decipher fruit quality, disease resistance, fruit morphology and reproductive barriers in melon/ MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ / MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/CEX2019-000902-S/ES/ / MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2021-128865NB-I00/ES/Validación y visualización de redes reguladoras del metabolismo especializado de plantas integrando métodos ómicos/ MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2017-23645/ES/ / FEDER/ / /EU/ / http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial 4.0 International Wiley