2026-04-14T03:25:32Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/30442025-10-22T11:29:34Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Almolda, Beatriz
author
Costa, Manuela
author
Montoya, Maria
author
González, Berta
author
Castellano, Bernardo
other
Producció Animal
group
Sanitat Animal
2025-10-22T11:29:34Z
2025-10-22T11:29:34Z
2011-11-07
Almolda, Beatriz, Manuela Costa, Maria Montoya, Berta González, and Bernardo Castellano. 2011. “Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate With the Recovery Phase of Acute EAE IN Rat.” PloS One 6 (11): e27473. doi: 10.1371/journal.pone.0027473
1932-6203
http://hdl.handle.net/20.500.12327/3044
https://doi.org/10.1371/journal.pone.0027473
Experimental autoimmune encephalomyelitis (EAE), a well-established model of multiple sclerosis, is characterised by
microglial activation and lymphocyte infiltration. Induction of EAE in Lewis rats produces an acute monophasic disease
characterised by a single peak of disability followed by a spontaneous and complete recovery and a subsequent tolerance
to further immunizations. In the current study we have performed a detailed analysis of the dynamics of different
lymphocyte populations and cytokine profile along the induction, peak, recovery and post-recovery phases in this
paradigm. MBP-injected rats were sacrificed attending exclusively to their clinical score, and the different populations of Tlymphocytes as well as the dynamics of different pro- and anti-inflammatory cytokines were analysed in the spinal cord by
flow cytometry, immunohistochemistry and ELISA. Our results revealed that, during the induction and peak phases, in
parallel to an increase in symptomatology, the number of CD3+ and CD4+ cells increased progressively, showing a Th1
phenotype, but unexpectedly during recovery, although clinical signs progressively decreased, the number and proportion
of CD3+ and CD4+ populations remained unaltered. Interestingly, during this recovery phase, we observed a marked
decrease of Th1 and an important increase in Th17 and T-reg cells. Moreover, our results indicate a specific cytokine
expression profile along the EAE course characterized by no changes of IL10 and IL17 levels, decrease of IL21 on the peak,
and high IL22 levels during the induction and peak phases that markedly decrease during recovery. In summary, these
results revealed the existence of a specific pattern of lymphocyte infiltration and cytokine secretion along the different
phases of the acute EAE model in Lewis rat that differs from those already described in chronic or relapsing-remitting mouse
models, where Th17-cells were found mostly during the peak, suggesting a specific role of these lymphocytes and cytokines
in the evolution of this acute EAE model.
eng
Attribution 4.0 International
Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat
info:eu-repo/semantics/article