2026-04-17T20:32:40Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/30432025-10-22T11:31:33Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Chaves, Aida J.
author
Busquets, Núria
author
Valle, Rosa
author
Rivas, Raquel
author
Serra Gironella, Joan
author
Dolz, Roser
author
Ramis, Antonio
author
Darji, Ayub
author
Majó, Natàlia
other
Producció Animal
group
Sanitat Animal
2025-10-22T11:31:33Z
2025-10-22T11:31:33Z
2011-10-07
Chaves, Aida J, Núria Busquets, Rosa Valle, Raquel Rivas, Júlia Vergara-Alert, Roser Dolz, Antonio Ramis, Ayub Darji, and Natàlia Majó. 2011. “Neuropathogenesis of a Highly Pathogenic Avian Influenza Virus (H7N1) in Experimentally Infected Chickens.” Veterinary Research 42 (1): 106. doi: 10.1186/1297-9716-42-106
0928-4249
http://hdl.handle.net/20.500.12327/3043
https://doi.org/10.1186/1297-9716-42-106
In order to understand the mechanism of neuroinvasion of a highly pathogenic avian influenza virus (HPAIV) into
the central nervous system (CNS) of chickens, specific pathogen free chickens were inoculated with a H7N1 HPAIV.
Blood, cerebrospinal fluid (CSF), nasal cavity and brain tissue samples were obtained from 1 to 4 days postinoculation (dpi) of infected and control chickens. Viral antigen topographical distribution, presence of influenza A
virus receptors in the brain, as well as, the role of the olfactory route in virus CNS invasion were studied using
different immunohistochemistry techniques. Besides, viral RNA load in CSF and blood was quantified by means of a
quantitative real-time reverse transcription-polymerase chain reaction. Viral antigen was observed widely distributed
in the CNS, showing bilateral and symmetrical distribution in the nuclei of the diencephalon, mesencephalon and
rhombencephalon. Viral RNA was detected in blood and CSF at one dpi, indicating that the virus crosses the
blood-CSF-barrier early during infection. This early dissemination is possibly favoured by the presence of Siaa2,3
Gal and Siaa2,6 Gal receptors in brain vascular endothelial cells, and Siaa2,3 Gal receptors in ependymal and
choroid plexus cells. No viral antigen was observed in olfactory sensory neurons, while the olfactory bulb showed
only weak staining, suggesting that the virus did not use this pathway to enter into the brain. The sequence of
virus appearance and the topographical distribution of this H7N1 HPAIV indicate that the viral entry occurs via the
haematogenous route, with early and generalized spreading through the CSF.
eng
Attribution 4.0 International
Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
info:eu-repo/semantics/article