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oai:recercat.cat:20.500.12327/29622025-10-22T11:00:07Zcom_2072_4428com_2072_4427col_2072_487898

Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells Mussá, Tufária Rodríguez-Cariño, Carolina Sánchez-Chardi, Alejandro Baratelli, Massimiliano Costa-Hurtado, Mar Fraile, Lorenzo Domínguez, Javier Aragon, Virginia Montoya, Maria Producció Animal Sanitat Animal Pigs possess a microbiota in the upper respiratory tract that includes Haemophilus parasuis. Pigs are also considered the reservoir of influenza viruses and infection with this virus commonly results in increased impact of bacterial infections, including those by H. parasuis. However, the mechanisms involved in host innate responses towards H. parasuis and their implications in a co-infection with influenza virus are unknown. Therefore, the ability of a non-virulent H. parasuis serovar 3 (SW114) and a virulent serovar 5 (Nagasaki) strains to interact with porcine bone marrow dendritic cells (poBMDC) and their modulation in a co-infection with swine influenza virus (SwIV) H3N2 was examined. At 1 hour post infection (hpi), SW114 interaction with poBMDC was higher than that of Nagasaki, while at 8 hpi both strains showed similar levels of interaction. The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone. Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki. These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection. 2025-10-22T11:00:07Z 2025-10-22T11:00:07Z 2025-10-22T11:00:07Z 2012-11-16 info:eu-repo/semantics/article Mussá, Tufária, Carolina Rodríguez-Cariño, Alejandro Sánchez-Chardi, Massimiliano Baratelli, Mar Costa-Hurtado, Lorenzo Fraile et al. 2012. “Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells”. Veterinary Research 43: 80. doi:10.1186/1297-9716-43-80 0928-4249 http://hdl.handle.net/20.500.12327/2962 https://doi.org/10.1186/1297-9716-43-80 eng Veterinary Research MEC/ /CSD2006-00007/ES/Patogenia de infecciones víricas del cerdo/PORCIVIR MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2009-12945-C02-01/ES/Vacunas Basadas En Capsidas Vacias (Vlps) Quimericas Frente Al Virus De La Gripe Porcina: Estudios Inmunologicos/ MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-22200-C02-01/ES/Caracterización de la respuesta inmune inducida por cepas del virus de la gripe porcina circulante en España. Desarrollo de vacunas basadas en VLPs quiméricas/ MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-15232/ES/Selección de candidatos vacunales para bloquear los pasos iniciales de la infección por Haemophilus parasuis/ http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Attribution 4.0 International BMC