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oai:recercat.cat:20.500.12327/29622025-10-22T11:00:07Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Mussá, Tufária author Rodríguez-Cariño, Carolina author Sánchez-Chardi, Alejandro author Baratelli, Massimiliano author Costa-Hurtado, Mar author Fraile, Lorenzo author Domínguez, Javier author Aragon, Virginia author Montoya, Maria author 2012-11-16 Pigs possess a microbiota in the upper respiratory tract that includes Haemophilus parasuis. Pigs are also considered the reservoir of influenza viruses and infection with this virus commonly results in increased impact of bacterial infections, including those by H. parasuis. However, the mechanisms involved in host innate responses towards H. parasuis and their implications in a co-infection with influenza virus are unknown. Therefore, the ability of a non-virulent H. parasuis serovar 3 (SW114) and a virulent serovar 5 (Nagasaki) strains to interact with porcine bone marrow dendritic cells (poBMDC) and their modulation in a co-infection with swine influenza virus (SwIV) H3N2 was examined. At 1 hour post infection (hpi), SW114 interaction with poBMDC was higher than that of Nagasaki, while at 8 hpi both strains showed similar levels of interaction. The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone. Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki. These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection. Mussá, Tufária, Carolina Rodríguez-Cariño, Alejandro Sánchez-Chardi, Massimiliano Baratelli, Mar Costa-Hurtado, Lorenzo Fraile et al. 2012. “Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells”. Veterinary Research 43: 80. doi:10.1186/1297-9716-43-80 0928-4249 http://hdl.handle.net/20.500.12327/2962 https://doi.org/10.1186/1297-9716-43-80 Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells