2026-04-17T01:24:45Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/28772025-10-22T11:17:29Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Torrealba, Débora
author
López, Daniela
author
Zelada, Patricio
author
Salinas-Parra, Nicolás
author
Valenzuela-Avilés, Paula
author
Garcia-Fruitós, Elena
author
Alomar, Oscar
author
Mercado, Luis
author
Altamirano, Claudia
author
Gallardo-Matus, José
other
Producció Animal
group
Producció de Remugants
2025-10-22T11:17:29Z
2025-10-22T11:17:29Z
2024-03-09
Torrealba, Débora, Daniela López, Patricio Zelada, Nicolás Salinas-Parra, Paula Valenzuela-Avilés, Elena Garcia-Fruitós, Anna Arís, Luis Mercado, Claudia Altamirano, and José Gallardo-Matus. 2024. “Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells.” Animals 14. doi:10.3390/ani14060844.
2076-2615
http://hdl.handle.net/20.500.12327/2877
https://doi.org/10.3390/ani14060844
Recent studies have demonstrated that immune-related recombinant proteins can enhance immune function, increasing host survival against infectious diseases in salmonids. This research evaluated inclusion bodies (IBs) of antimicrobial peptides (CAMPIB and HAMPIB) and a cytokine (IL1βIB and TNFαIB) as potential immunostimulants in farmed salmonids. For this purpose, we produced five IBs (including iRFPIB as a control), and we evaluated their ability to modulate immune marker gene expression of three IBs in the RTS11 cell line by RT–qPCR. Additionally, we characterized the scale-up of IBs production by comparing two different scale systems. The results showed that CAMPIB can increase the upregulation of tnfα, il1β, il8, and il10, HAMPIB significantly increases the upregulation of tnfα, inos, and il10, and IL1βIB significantly upregulated the expression of tnfα, il1β, and cox2. A comparison of IL1βIB production showed that the yield was greater in shake flasks than in bioreactors (39 ± 1.15 mg/L and 14.5 ± 4.08 mg/L), and larger nanoparticles were produced in shake flasks (540 ± 129 nm and 427 ± 134 nm, p < 0.0001, respectively). However, compared with its shake flask counterpart, the IL1βIB produced in a bioreactor has an increased immunomodulatory ability. Further studies are needed to understand the immune response pathways activated by IBs and the optimal production conditions in bioreactors, such as a defined medium, fed-batch production, and mechanical bacterial lysis, to increase yield.
eng
Attribution 4.0 International
Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells
info:eu-repo/semantics/article