2026-04-13T02:57:26Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/28172025-10-22T11:20:15Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
López-Figueroa, Carlos
author
Cano, Esmeralda
author
Navarro, Núria
author
Pérez-Maíllo, Mónica
author
Pujols, Joan
author
Núñez, José I.
author
Serra Gironella, Joan
author
Segalés, Joaquim
other
Producció Animal
group
Sanitat Animal
2025-10-22T11:20:15Z
2025-10-22T11:20:15Z
2023-12-27
López-Figueroa, Carlos, Esmeralda Cano, Núria Navarro, Mónica Pérez-Maíllo, Joan Pujols, José I. Núñez, Júlia Vergara‐Alert, and Joaquím Segalés. 2023. “Clinical, Pathological and Virological Outcomes of Tissue-Homogenate-Derived and Cell-Adapted Strains of Porcine Epidemic Diarrhea Virus (PEDV) in a Neonatal Pig Model.” Viruses 16 (1): 44. doi:org/10.3390/v16010044.
1999-4915
http://hdl.handle.net/20.500.12327/2817
https://doi.org/10.3390/v16010044
Porcine epidemic diarrhea virus (PEDV) is characterized by diarrhea, vomiting, dehydration, and high mortality rates in neonatal piglets. Two distinct genogroups, S-INDEL (G1a, G1b) and
non-S INDEL (G2a, G2b, and G2c), circulate worldwide and are characterized by varying degrees
of virulence. Here, we compared the early pathogenesis of a PEDV S-INDEL strain obtained from
intestine homogenate (CALAF-HOMOG) or adapted to cell culture by 22 passages (CALAF-ADAP)
and a virulent non-S INDEL strain (PEDV-USA) in newborn piglets. After orogastric inoculation of
PEDV strains, body weight, temperature and clinical signs were monitored for 48 hpi. Pathological
studies were performed at 48 hpi and RNA extracts from jejunal content (at 48 hpi) and rectal swabs
(at 0 and 48 hpi) were tested for the presence of PEDV RNA as well as sequenced and compared
to the inoculum. Piglets inoculated with PEDV-USA and CALAF-HOMOG isolates showed more
severe weight loss, diarrhea, villi fusion and atrophy compared to CALAF-ADAP inoculated piglets.
The viral load of rectal swabs was higher in the PEDV-USA inoculated group, followed by CALAFHOMOG and CALAF-ADAP isolates. Similarly, viral RNA load in jejunal content was comparable
among PEDV-USA and CALAF-HOMOG inoculated piglets and higher than that of CALAF-ADAP
ones. The comparison of three full PEDV sequences of the inocula with the corresponding ones of
pigs after 48 hpi yielded a nucleotide identity >99.9%. This study highlights variations in virulence
among S-INDEL and non-S INDEL strains and between S-INDEL isolates obtained from homogenate
and cell culture.
eng
Attribution 4.0 International
Clinical, Pathological and Virological Outcomes of Tissue-Homogenate-Derived and Cell-Adapted Strains of Porcine Epidemic Diarrhea Virus (PEDV) in a Neonatal Pig Model
info:eu-repo/semantics/article