2026-04-18T05:59:31Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/27102025-10-22T11:31:19Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Kikuti, Mariana
author
Vilalta, Carles
author
Sanhueza, Juan
author
Pamornchainavakul, Nakarin
author
Kevill, Jessica
author
Yang, My
author
Paploski, Igor A. D.
author
Lenskaia, Tatiana
author
Odogwu, Nkechi M.
author
Kiehne, Ross
author
VanderWaal, Kimberly
author
Schroeder, Declan
author
Corzo, Cesar A.
other
Producció Animal
group
Sanitat Animal
2025-10-22T11:31:19Z
2025-10-22T11:31:19Z
2023-08-30
Kikuti, Mariana, Carles Vilalta, Juan Sanhueza, Nakarin Pamornchainavakul, Jessica L. Kevill, Ming‐Chien Yang, Igor Adolfo Dexheimer Paploski, et al. 2023 “Porcine Reproductive and Respiratory Syndrome (PRRSV2) Viral Diversity within a Farrow-to-Wean Farm Cohort Study.” Viruses 15 (9): 1837. doi:10.3390/v15091837.
1999-4915
http://hdl.handle.net/20.500.12327/2710
https://doi.org/10.3390/v15091837
Describing PRRSV whole-genome viral diversity data over time within the host and withinfarm is crucial for a better understanding of viral evolution and its implications. A cohort study was
conducted at one naïve farrow-to-wean farm reporting a PRRSV outbreak. All piglets 3–5 days of age
(DOA) born to mass-exposed sows through live virus inoculation with the recently introduced wildtype virus two weeks prior were sampled and followed up at 17–19 DOA. Samples from 127 piglets
were individually tested for PRRSV by RT-PCR and 100 sequences were generated using Oxford
Nanopore Technologies chemistry. Female piglets had significantly higher median Ct values than
males (15.5 vs. 13.7, Kruskal–Wallis p < 0.001) at 3–5 DOA. A 52.8% mortality between sampling
points was found, and the odds of dying by 17–19 DOA decreased with every one unit increase
in Ct values at 3–5 DOA (OR = 0.76, 95% CI 0.61–0.94, p = 0.01). Although the within-pig percent
nucleotide identity was overall high (99.7%) between 3–5 DOA and 17–19 DOA samples, ORFs 4
and 5a showed much lower identities (97.26% and 98.53%, respectively). When looking solely at
ORF5, 62% of the sequences were identical to the 3–5 DOA consensus. Ten and eight regions showed
increased nucleotide and amino acid genetic diversity, respectively, all found throughout ORFs 2a/2b,
4, 5a/5, 6, and 7.
eng
Attribution 4.0 International
Porcine Reproductive and Respiratory Syndrome (PRRSV2) Viral Diversity within a Farrow-to-Wean Farm Cohort Study
info:eu-repo/semantics/article