2026-04-18T02:11:09Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/27102025-10-22T11:31:19Zcom_2072_4428com_2072_4427col_2072_487898

2025-10-22T11:31:19Z urn:hdl:20.500.12327/2710 Porcine Reproductive and Respiratory Syndrome (PRRSV2) Viral Diversity within a Farrow-to-Wean Farm Cohort Study Kikuti, Mariana Vilalta, Carles Sanhueza, Juan Pamornchainavakul, Nakarin Kevill, Jessica Yang, My Paploski, Igor A. D. Lenskaia, Tatiana Odogwu, Nkechi M. Kiehne, Ross VanderWaal, Kimberly Schroeder, Declan Corzo, Cesar A. Producció Animal Sanitat Animal Describing PRRSV whole-genome viral diversity data over time within the host and withinfarm is crucial for a better understanding of viral evolution and its implications. A cohort study was conducted at one naïve farrow-to-wean farm reporting a PRRSV outbreak. All piglets 3–5 days of age (DOA) born to mass-exposed sows through live virus inoculation with the recently introduced wildtype virus two weeks prior were sampled and followed up at 17–19 DOA. Samples from 127 piglets were individually tested for PRRSV by RT-PCR and 100 sequences were generated using Oxford Nanopore Technologies chemistry. Female piglets had significantly higher median Ct values than males (15.5 vs. 13.7, Kruskal–Wallis p < 0.001) at 3–5 DOA. A 52.8% mortality between sampling points was found, and the odds of dying by 17–19 DOA decreased with every one unit increase in Ct values at 3–5 DOA (OR = 0.76, 95% CI 0.61–0.94, p = 0.01). Although the within-pig percent nucleotide identity was overall high (99.7%) between 3–5 DOA and 17–19 DOA samples, ORFs 4 and 5a showed much lower identities (97.26% and 98.53%, respectively). When looking solely at ORF5, 62% of the sequences were identical to the 3–5 DOA consensus. Ten and eight regions showed increased nucleotide and amino acid genetic diversity, respectively, all found throughout ORFs 2a/2b, 4, 5a/5, 6, and 7. 2025-10-22T11:31:19Z 2025-10-22T11:31:19Z 2023-08-30 info:eu-repo/semantics/article Kikuti, Mariana, Carles Vilalta, Juan Sanhueza, Nakarin Pamornchainavakul, Jessica L. Kevill, Ming‐Chien Yang, Igor Adolfo Dexheimer Paploski, et al. 2023 “Porcine Reproductive and Respiratory Syndrome (PRRSV2) Viral Diversity within a Farrow-to-Wean Farm Cohort Study.” Viruses 15 (9): 1837. doi:10.3390/v15091837. 1999-4915 http://hdl.handle.net/20.500.12327/2710 https://doi.org/10.3390/v15091837 eng Viruses http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Attribution 4.0 International MDPI