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Variability in glutenin subunit composition of Mediterranean durum wheat germplasm and its relationship with gluten strength Nazco, R. Peña, R.J. Ammar, K. Villegas, D. Crossa, J. Moragues, M. Royo, C. Producció Vegetal Cultius Extensius Sostenibles 633 The allelic composition at five glutenin loci was assessed by one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS–PAGE) on a set of 155 landraces (from 21 Mediterranean countries) and 18 representative modern varieties. Gluten strength was determined by SDS-sedimentation on samples grown under rainfed conditions during 3 years in north-eastern Spain. One hundred and fourteen alleles/banding patterns were identified (25 at Glu-1 and 89 at Glu-2/Glu-3 loci); 0·85 of them were in landraces at very low frequency and 0·72 were unreported. Genetic diversity index was 0·71 for landraces and 0·38 for modern varieties. All modern varieties exhibited medium to strong gluten type with none of their 13 banding patterns having a significant effect on gluten-strength type. Ten banding patterns significantly affected gluten strength in landraces. Alleles Glu-B1e (band 20), Glu-A3a (band 6), Glu-A3d (bands 6+11), Glu-B3a (bands 2+4+15+19) and Glu-B2a (band 12) significantly increased the SDS-value, and their effects were associated with their frequency. Two alleles, Glu-A3b (band 5) and Glu-B2b (null), significantly reduced gluten strength, but only the effect of the latter locus could be associated with its frequency. Only three rare banding patterns affected gluten strength significantly: Glu-B1a (band 7), found in six landraces, had a negative effect, whereas banding patterns 2+4+14+15+18 and 2+4+15+18+19 at Glu-B3 had a positive effect. Landraces with outstanding gluten strength were more frequent in eastern than in western Mediterranean countries. The geographical pattern displayed from the frequencies of Glu-A1c is discussed. R. Nazco was recipient of a Ph.D. grant from the Comissionat per Universitats i Investigació del Departament d’Innovació, Universitats i Empresa of the Generalitat of Catalonia and the Fondo Social Europeo. This study was partially funded by CICYT under projects AGL2006-09226-C02-01, AGL2009- 11187 and AGL2012-37217 and was developed within the framework of the agreement between INIA Spain and CIMMYT. The Centre UdL-IRTA is part of the Centre CONSOLIDER INGENIO 2010 on Agrigenomics funded by the Spanish Ministry of Education and Science. CRF, ICARDA and USDA Germplasm Bank are acknowledged for providing accessions for the present study. info:eu-repo/semantics/publishedVersion 2013-01-23 info:eu-repo/semantics/article Nazco, R., R. J. Peña, K. Ammar, D. Villegas, J. Crossa, M. Moragues, and C. Royo. 2013. "Variability In Glutenin Subunit Composition Of Mediterranean Durum Wheat Germplasm And Its Relationship With Gluten Strength". The Journal Of Agricultural Science 152 (3): 379-393. doi:10.1017/s0021859613000117. 0021-8596 http://hdl.handle.net/20.500.12327/2357 https://doi.org/10.1017/S0021859613000117 eng Cambridge University Press MEC/Programa nacional de recursos y tecnologías agroalimentarias/AGL2006-09226-C02-01/ES/NUEVAS VIAS PARA MEJORAR LA ADAPTACION DEL TRIGO DURO, TRITICUM TURGIDUM L. VAR. DURUM, A AMBIENTES MEDITERRANEOS/ MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2009-11187/ES/Componentes De Tolerancia A La Sequia E Impacto En Mejora De Trigo: Bases Genetica Y Molecular Del Contenido De Chl/ MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-37217/ES/HERRAMIENTAS PARA LA MEJORA DEL TRIGO DURO FRENTE A LOS RETOS DEL CAMBIO CLIMATICO/ Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ info:eu-repo/semantics/openAccess 15 Cambridge University Press