2026-04-14T03:33:12Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/23572025-10-22T11:32:28Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Nazco, R.
author
Peña, R.J.
author
Ammar, K.
author
Villegas, D.
author
Crossa, J.
author
Moragues, M.
author
Royo, C.
other
Producció Vegetal
group
Cultius Extensius Sostenibles
2025-10-22T11:32:28Z
2025-10-22T11:32:28Z
2013-01-23
Nazco, R., R. J. Peña, K. Ammar, D. Villegas, J. Crossa, M. Moragues, and C. Royo. 2013. "Variability In Glutenin Subunit Composition Of Mediterranean Durum Wheat Germplasm And Its Relationship With Gluten Strength". The Journal Of Agricultural Science 152 (3): 379-393. doi:10.1017/s0021859613000117.
0021-8596
http://hdl.handle.net/20.500.12327/2357
https://doi.org/10.1017/S0021859613000117
The allelic composition at five glutenin loci was assessed by one-dimensional sodium dodecyl sulphate
polyacrylamide gel electrophoresis (1D SDS–PAGE) on a set of 155 landraces (from 21 Mediterranean countries)
and 18 representative modern varieties. Gluten strength was determined by SDS-sedimentation on samples grown
under rainfed conditions during 3 years in north-eastern Spain. One hundred and fourteen alleles/banding patterns
were identified (25 at Glu-1 and 89 at Glu-2/Glu-3 loci); 0·85 of them were in landraces at very low frequency and
0·72 were unreported. Genetic diversity index was 0·71 for landraces and 0·38 for modern varieties. All modern
varieties exhibited medium to strong gluten type with none of their 13 banding patterns having a significant effect
on gluten-strength type. Ten banding patterns significantly affected gluten strength in landraces. Alleles Glu-B1e
(band 20), Glu-A3a (band 6), Glu-A3d (bands 6+11), Glu-B3a (bands 2+4+15+19) and Glu-B2a (band 12)
significantly increased the SDS-value, and their effects were associated with their frequency. Two alleles, Glu-A3b
(band 5) and Glu-B2b (null), significantly reduced gluten strength, but only the effect of the latter locus could be
associated with its frequency. Only three rare banding patterns affected gluten strength significantly: Glu-B1a
(band 7), found in six landraces, had a negative effect, whereas banding patterns 2+4+14+15+18 and
2+4+15+18+19 at Glu-B3 had a positive effect. Landraces with outstanding gluten strength were more frequent
in eastern than in western Mediterranean countries. The geographical pattern displayed from the frequencies of
Glu-A1c is discussed.
eng
Attribution-NonCommercial-ShareAlike 4.0 International
Variability in glutenin subunit composition of Mediterranean durum wheat germplasm and its relationship with gluten strength
info:eu-repo/semantics/article