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oai:recercat.cat:20.500.12327/22562025-10-22T11:10:58Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT author Rodriguez, Ariel author Falcon, Ana author Cuevas, Maria Teresa author Pozo, Francisco author Guerra, Susana author García-Barreno, Blanca author Martinez-Orellana, Pamela author Pérez-Breña, Pilar author Montoya, Maria author Melero, Jose Antonio author Ortin, Juan author Casas, Inmaculada author Nieto, Amelia other Producció Animal group Sanitat Animal 2025-10-22T11:10:58Z 2025-10-22T11:10:58Z 2013-01-10 Rodriguez, Ariel, Ana Falcon, Maria Teresa Cuevas, Francisco Pozo, Susana Guerra, Blanca García-Barreno, and Pamela Martinez-Orellana et al. 2013. "Characterization In Vitro And In Vivo Of A Pandemic H1N1 Influenza Virus From A Fatal Case". Plos ONE 8 (1): e53515. doi:10.1371/journal.pone.0053515. 1932-6203 http://hdl.handle.net/20.500.12327/2256 https://doi.org/10.1371/journal.pone.0053515 Pandemic 2009 H1N1 (pH1N1) influenza viruses caused mild symptoms in most infected patients. However, a greater rate of severe disease was observed in healthy young adults and children without co-morbid conditions. Here we tested whether influenza strains displaying differential virulence could be present among circulating pH1N1 viruses. The biological properties and the genotype of viruses isolated from a patient showing mild disease (M) or from a fatal case (F), both without known co-morbid conditions were compared in vitro and in vivo. The F virus presented faster growth kinetics and stronger induction of cytokines than M virus in human alveolar lung epithelial cells. In the murine model in vivo, the F virus showed a stronger morbidity and mortality than M virus. Remarkably, a higher proportion of mice presenting infectious virus in the hearts, was found in F virus-infected animals. Altogether, the data indicate that strains of pH1N1 virus with enhanced pathogenicity circulated during the 2009 pandemic. In addition, examination of chemokine receptor 5 (CCR5) genotype, recently reported as involved in severe influenza virus disease, revealed that the F virus-infected patient was homozygous for the deleted form of CCR5 receptor (CCR5D32) eng Attribution 4.0 International Characterization In Vitro and In Vivo of a Pandemic H1N1 Influenza Virus from a Fatal Case info:eu-repo/semantics/article