2026-04-13T11:37:00Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/21782025-10-22T11:29:24Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Moro-Martínez, Irene
author
Vázquez-Luis, Maite
author
García-March, José Rafael
author
Prado, Patricia
author
Mičić, Milena
author
Catanese, Gaetano
other
Producció Animal
group
Aigües Marines i Continentals
2025-10-22T11:29:24Z
2025-10-22T11:29:24Z
2023-04-28
Moro-Martínez, Irene, Maite Vázquez-Luis, José Rafael García-March, Patricia Prado, Milena Mičić, and Gaetano Catanese. 2023. "Haplosporidium Pinnae Parasite Detection In Seawater Samples". Microorganisms 11 (5): 1146. https://www.mdpi.com/2076-2607/11/5/1146.
2076-2607
http://hdl.handle.net/20.500.12327/2178
https://doi.org/10.3390/microorganisms11051146
In this study, we investigated the presence of the parasite Haplosporidium pinnae, which is a pathogen for the bivalve Pinna nobilis, in water samples from different environments. Fifteen mantle samples of P. nobilis infected by H. pinnae were used to characterize the ribosomal unit of this parasite. The obtained sequences were employed to develop a method for eDNA detection of H. pinnae. We collected 56 water samples (from aquaria, open sea and sanctuaries) for testing the methodology. In this work, we developed three different PCRs generating amplicons of different lengths to determine the level of degradation of the DNA, since the status of H. pinnae in water and, therefore, its infectious capacity are unknown. The results showed the ability of the method to detect H. pinnae in sea waters from different areas persistent in the environment but with different degrees of DNA fragmentation. This developed method offers a new tool for preventive analysis for monitoring areas and to better understand the life cycle and the spread of this parasite.
eng
Attribution 4.0 International
Haplosporidium pinnae Parasite Detection in Seawater Samples
info:eu-repo/semantics/article