2026-04-13T13:28:59Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/21782025-10-22T11:29:24Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Moro-Martínez, Irene author Vázquez-Luis, Maite author García-March, José Rafael author Prado, Patricia author Mičić, Milena author Catanese, Gaetano author 2023-04-28 In this study, we investigated the presence of the parasite Haplosporidium pinnae, which is a pathogen for the bivalve Pinna nobilis, in water samples from different environments. Fifteen mantle samples of P. nobilis infected by H. pinnae were used to characterize the ribosomal unit of this parasite. The obtained sequences were employed to develop a method for eDNA detection of H. pinnae. We collected 56 water samples (from aquaria, open sea and sanctuaries) for testing the methodology. In this work, we developed three different PCRs generating amplicons of different lengths to determine the level of degradation of the DNA, since the status of H. pinnae in water and, therefore, its infectious capacity are unknown. The results showed the ability of the method to detect H. pinnae in sea waters from different areas persistent in the environment but with different degrees of DNA fragmentation. This developed method offers a new tool for preventive analysis for monitoring areas and to better understand the life cycle and the spread of this parasite. Moro-Martínez, Irene, Maite Vázquez-Luis, José Rafael García-March, Patricia Prado, Milena Mičić, and Gaetano Catanese. 2023. "Haplosporidium Pinnae Parasite Detection In Seawater Samples". Microorganisms 11 (5): 1146. https://www.mdpi.com/2076-2607/11/5/1146. 2076-2607 http://hdl.handle.net/20.500.12327/2178 https://doi.org/10.3390/microorganisms11051146 Haplosporidium pinnae Parasite Detection in Seawater Samples