2026-04-13T05:03:06Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/21282025-10-22T11:06:23Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Real, Núria
author
Villar, Irene
author
Serrano, Irene
author
Guiu-Aragonés, Cèlia
author
Martin-Hernandez, Ana Montserrat
other
Producció Vegetal
group
Genòmica i Biotecnologia
2025-10-22T11:06:23Z
2025-10-22T11:06:23Z
2022-12-17
Real, Núria, Irene Villar, Irene Serrano, Cèlia Guiu-Aragonés, and Ana Montserrat Martín-Hernández. 2022. "Mutations In Cmvps41 Controlling Resistance To Cucumber Mosaic Virus Display Specific Subcellular Localization". Plant Physiology. doi:10.1093/plphys/kiac583.
0032-0889
http://hdl.handle.net/20.500.12327/2128
https://doi.org/10.1093/plphys/kiac583
Resistance to cucumber mosaic virus (CMV) in melon (Cucumis melo L.) has been described in several exotic accessions and is controlled by a recessive resistance gene, cmv1, that encodes a vacuolar protein sorting 41 (CmVPS41). cmv1 prevents systemic infection by restricting the virus to the bundle sheath cells, preventing viral phloem entry. CmVPS41 from different resistant accessions carries two causal mutations, either a G85E change, found in Pat-81 and Freeman’s cucumber, or L348R, found in PI161375, cultivar Songwhan Charmi (SC). Here, we analyzed the subcellular localization of CmVPS41 in Nicotiana benthamiana and found differential structures in resistant and susceptible accessions. Susceptible accessions showed nuclear and membrane spots and many transvacuolar strands, whereas the resistant accessions showed many intravacuolar invaginations. These specific structures colocalized with late endosomes. Artificial CmVPS41 carrying individual mutations causing resistance in the genetic background of CmVPS41 from the susceptible variety Piel de Sapo (PS) revealed that the structure most correlated with resistance was the absence oftransvacuolar strands. Coexpression of CmVPS41 with viral movement proteins, the determinant of virulence, did not change these localizations; however, infiltration of CmVPS41 from either SC or PS accessions in CMV-infected N. benthamiana leaves showed a localization pattern closerto each other, with upto 30% cellsshowingsomemembranespots inthe CmVPS41SC and fewertransvacuolarstrands (reduced from a mean of 4 to 1–2) with CmVPS41PS. Our results suggest that the distribution of CmVPS41PS in late endosomes includes transvacuolar strands that facilitate CMV infection and that CmVPS41 re-localizes during viral infection.
eng
Copyright © 2022, Oxford University Press
Mutations in CmVPS41 controlling resistance to cucumber mosaic virus display specific subcellular localization
info:eu-repo/semantics/article