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00925njm 22002777a 4500 dc Fernández-Bastit, Leira author Roca, Núria author Romero-Durana, Miguel author Rodon Aldrufeu, Jordi author Cantero Portillo, Guillermo author García, Óscar author López, Carlos author Pérez, Mònica author López, Rosa author Carrillo, Jorge author Izquierdo-Useros, Nuria author Blanco, Julià author Clotet, Bonaventura author Pujols, Joan author Vergara-Alert, Júlia author Segalés, Joaquim author Lorca-Oró, Cristina author 2022-09-09 A wide range of animal species are susceptible to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Natural and/or experimental infections have been reported in pet, zoo, farmed and wild animals. Interestingly, some SARS-CoV-2 variants, such as B.1.1.7/Alpha, B.1.351/Beta, and B.1.1.529/Omicron, were demonstrated to infect some animal species not susceptible to classical viral variants. The present study aimed to elucidate if goats (Capra aegagrus hircus) are susceptible to the B.1.351/Beta variant. First, an in silico approach was used to predict the affinity between the receptor-binding domain of the spike protein of SARS-CoV-2 B.1.351/Beta variant and angiotensin-converting enzyme 2 from goats. Moreover, we performed an experimental inoculation with this variant in domestic goat and showed evidence of infection. SARS-CoV-2 was detected in nasal swabs and tissues by RT-qPCR and/or immunohistochemistry, and seroneutralisation was confirmed via ELISA and live virus neutralisation assays. However, the viral amount and tissue distribution suggest a low susceptibility of goats to the B.1.351/Beta variant. Therefore, although monitoring livestock is advisable, it is unlikely that goats play a role as SARS-CoV-2 reservoir species, and they are not useful surrogates to study SARS-CoV-2 infection in farmed animals. Fernández-Bastit, Leira, Núria Roca, Miguel Romero-Durana, Jordi Rodon, Guillermo Cantero, Óscar García, Carlos López, Mònica Pérez, Rosa López, Jorge Carrillo, Nuria Izquierdo-Useros, Julià Blanco, Bonaventura Clotet, Joan Pujols, Júlia Vergara-Alert, Joaquim Segalés, and Cristina Lorca-Oró. 2022. "Susceptibility Of Domestic Goat (Capra Aegagrus Hircus) To Experimental Infection With Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-Cov-2) B.1.351/Beta Variant". Viruses 14 (9): 2002. doi:10.3390/v14092002. 1999-4915 http://hdl.handle.net/20.500.12327/2069 https://doi.org/10.3390/v14092002 Susceptibility of Domestic Goat (Capra aegagrus hircus) to Experimental Infection with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) B.1.351/Beta Variant