2026-04-13T04:58:33Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/19872025-10-22T11:19:36Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Bosch Camós, Laia author Alonso, Uxía author Esteve-Codina, Anna author Chang, Chia-Yu author Martín-Mur, Beatriz author Accensi, Francesc author Muñoz, Marta author Navas, María J. author Dabad, Marc author Vidal, Enric author Pina-Pedrero, Sonia author Pleguezuelos, Patricia author Caratù, Ginevra author Salas, María L. author Liu, Lihong author Bataklieva, Stanimira author Gavrilov, Boris author Rodriguez, Fernando author Argilaguet, Jordi author 2022-11-09 African swine fever virus (ASFV) is causing a worldwide pandemic affecting the porcine industry and leading to important global economic consequences. The virus causes a highly lethal hemorrhagic disease in wild boars and domestic pigs. Lack of effective vaccines hampers the control of virus spread, thus increasing the pressure on the scientific community for urgent solutions. However, knowledge on the immune components associated with protection is very limited. Here we characterized the in vitro recall response induced by immune cells from pigs intranasally vaccinated with the BA71ΔCD2 deletion mutant virus. Vaccination conferred dose-dependent cross-protection associated with both ASFV-specific antibodies and IFNγ-secreting cells. Importantly, bulk and single-cell transcriptomics of blood and lymph node cells from vaccinated pigs revealed a positive feedback from adaptive to innate immunity. Indeed, activation of Th1 and cytotoxic T cells was concomitant with a rapid IFNγ-dependent triggering of an inflammatory response characterized by TNF-producing macrophages, as well as CXCL10-expressing lymphocytes and cross-presenting dendritic cells. Altogether, this study provides a detailed phenotypic characterization of the immune cell subsets involved in cross-protection against ASFV, and highlights key functional immune mechanisms to be considered for the development of an effective ASF vaccine. Bosch-Camós, Laia, Uxía Alonso, Anna Esteve-Codina, Chia-Yu Chang, Beatriz Martín-Mur, Francesc Accensi, and Marta Muñoz et al. 2022. "Cross-Protection Against African Swine Fever Virus Upon Intranasal Vaccination Is Associated With An Adaptive-Innate Immune Crosstalk". PLOS Pathogens 18 (11): e1010931. doi:10.1371/journal.ppat.1010931. 1553-7366 http://hdl.handle.net/20.500.12327/1987 https://doi.org/10.1371/journal.ppat.1010931 Cross-protection against African swine fever virus upon intranasal vaccination is associated with an adaptive-innate immune crosstalk