2026-04-14T06:43:08Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/19402025-10-22T11:30:26Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Peñarrubia, Luis author Alcaraz, Carles author de Vaate, Abraham bij author Sanz, Nuria author Pla, Carles author Vidal, Oriol author Viñas, Jordi author 2016-12-14 The zebra mussel (Dreissena polymorpha Pallas, 1771) and the quagga mussel (D. rostriformis Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locations. Peñarrubia, Luis, Carles Alcaraz, Abraham bij de Vaate, Nuria Sanz, Carles Pla, Oriol Vidal, and Jordi Viñas. 2016. "Validated Methodology For Quantifying Infestation Levels Of Dreissenid Mussels In Environmental DNA (Edna) Samples". Scientific Reports 6 (1). doi:10.1038/srep39067. 2045-2322 http://hdl.handle.net/20.500.12327/1940 https://doi.org/10.1038/srep39067 Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples