2026-04-18T00:14:39Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/19262025-10-22T11:22:53Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Alkassar, Mounira author Leonardo, Sandra author Diogène, Jorge author Campàs, Mònica author 2022-09-24 Marine toxins are potent toxic compounds that may reach humans and poison them. Therefore, their detection in seafood is crucial to prevent intoxication cases. Colorimetric cell-based assays (CBAs) have been developed to analyse marine neurotoxins, such as ciguatoxins (CTXs) and tetrodotoxins (TTXs), and are based on the toxicological effect of these toxins on the cells. Cell viability can be quantified by measuring the mitochondrial activity with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). With the purpose of moving forward in the development of cell-based biosensors (CBBs) for neurotoxins, Neuro-2a cells were immobilised on electrodes of different materials (carbon, carbon/polyaniline, carbon/poly-L-lysine, carbon/poly(3,4-ethylenedioxythiophene) and gold) and their presence and viability were assessed by the detection of MTT formazan crystals with cyclic voltammetry. Best results in terms of oxidation potential and current intensity were achieved with carbon and carbon/polyaniline electrodes. Light microscopy also proved the presence of immobilised and living cells on electrodes. Cell density, incubation time and MTT concentration were optimised. Appropriate electrochemical responses were obtained incubating 100,000 cells/electrode for 2 h and using 0.86 mg/mL MTT. The system was able to detect toxicity when exposed to CTX1B and TTX standard solutions as well as Seriola dumerili and Lagocephalus sceleratus fish extracts containing these toxins. Alkassar, Mounira, Sandra Leonardo, Jorge Diogène, and Mònica Campàs. 2022. "Immobilisation Of Neuro-2A Cells On Electrodes And Electrochemical Detection Of MTT Formazan Crystals To Assess Their Viability". Bioelectrochemistry, 108274. doi:10.1016/j.bioelechem.2022.108274. 1567-5394 http://hdl.handle.net/20.500.12327/1926 https://doi.org/10.1016/j.bioelechem.2022.108274 Immobilisation of Neuro-2a cells on electrodes and electrochemical detection of MTT formazan crystals to assess their viability