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oai:recercat.cat:20.500.12327/18712025-10-22T11:04:38Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Nimmakayala, Padma author Tomason, Yan R. author Abburi, Venkata L. author Alvarado, Alejandra author Saminathan, Thangasamy author Vajja, Venkata G. author Salazar, Germania author Panicker, Girish K. author Levi, Amnon author Wechter, William P. author McCreight, James D. author Korol, Abraham B. author Ronin, Yefim author Garcia-Mas, Jordi author Reddy, Umesh K. author 2016-09-22 Melon (Cucumis melo L.) is a phenotypically diverse eudicot diploid (2n = 2x = 24) has climacteric and non-climacteric morphotypes and show wide variation for fruit firmness, an important trait for transportation and shelf life. We generated 13,789 SNP markers using genotyping-by-sequencing (GBS) and anchored them to chromosomes to understand genome-wide fixation indices (Fst) between various melon morphotypes and genomewide linkage disequilibrium (LD) decay. The FST between accessions of cantalupensis and inodorus was 0.23. The FST between cantalupensis and various agrestis accessions was in a range of 0.19–0.53 and between inodorus and agrestis accessions was in a range of 0.21–0.59 indicating sporadic to wide ranging introgression. The EM (Expectation Maximization) algorithm was used for estimation of 1436 haplotypes. Average genome-wide LD decay for the melon genome was noted to be 9.27 Kb. In the current research, we focused on the genome-wide divergence underlying diverse melon horticultural groups. A high-resolution genetic map with 7153 loci was constructed. Genome-wide segregation distortion and recombination rate across various chromosomes were characterized. Melon has climacteric and non-climacteric morphotypes and wide variation for fruit firmness, a very important trait for transportation and shelf life. Various levels of QTLs were identified with high to moderate stringency and linked to fruit firmness using both genome-wide association study (GWAS) and biparental mapping. Gene annotation revealed some of the SNPs are located in β-D-xylosidase, glyoxysomal malate synthase, chloroplastic anthranilate phosphoribosyltransferase, and histidine kinase, the genes that were previously characterized for fruit ripening and softening in other crops. Nimmakayala, Padma, Yan R. Tomason, Venkata L. Abburi, Alejandra Alvarado, Thangasamy Saminathan, Venkata G. Vajja, and Germania Salazar et al. 2016. "Genome-Wide Differentiation Of Various Melon Horticultural Groups For Use In GWAS For Fruit Firmness And Construction Of A High Resolution Genetic Map". Frontiers In Plant Science 7. doi:10.3389/fpls.2016.01437. 1664-462X http://hdl.handle.net/20.500.12327/1871 https://doi.org/10.3389/fpls.2016.01437 Genome-Wide Differentiation of Various Melon Horticultural Groups for Use in GWAS for Fruit Firmness and Construction of a High Resolution Genetic Map