2026-04-14T04:03:50Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/18442025-10-22T11:17:36Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Cano-Garrido, Olivia author Céspedes, María Virtudes author Unzueta, Ugutz author Saccardo, Paolo author Roldán, Mònica author Sánchez-Chardi, Alejandro author Cubarsi, Rafael author Vázquez, Esther author Mangues, Ramon author García-Fruitós, Elena author Villaverde, Antonio author 2016-08-16 Aim: Lactococcus lactis is a Gram-positive (endotoxin-free) food-grade bacteria exploited as alternative to Escherichia coli for recombinant protein production. We have explored here for the first time the ability of this platform as producer of complex, self-assembling protein materials. Materials & methods: Biophysical properties, cell penetrability and in vivo biodistribution upon systemic administration of tumor-targeted protein nanoparticles produced in L. lactis have been compared with the equivalent material produced in E. coli. Results: Protein nanoparticles have been efficiently produced in L. lactis, showing the desired size, internalization properties and biodistribution. Conclusion: In vitro and in vivo data confirm the potential and robustness of the production platform, pointing out L. lactis as a fascinating cell factory for the biofabrication of protein materials intended for therapeutic applications. Cano-Garrido, Olivia, María Virtudes Céspedes, Ugutz Unzueta, Paolo Saccardo, Mònica Roldán, Alejandro Sánchez-Chardi, Rafael Cubarsi, Esther Vázquez, Ramon Mangues, Elena García-Fruitós and Antonio Villaverde. 2016. "CXCR4+-Targeted Protein Nanoparticles Produced In The Food-Grade Bacterium Lactococcus Lactis | Nanomedicine". Nanomedicine 11 (18): 2387-2398. https://www.futuremedicine.com/doi/10.2217/nnm-2016-0200. 1743-5889 http://hdl.handle.net/20.500.12327/1844 https://doi.org/10.2217/nnm-2016-0200 CXCR4+-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis