2026-04-18T05:20:11Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/1802025-10-22T11:20:09Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Herrera-Uribe, Júber
author
Jiménez-Marín, Ángeles
author
Lacasta, Anna
author
L. Monteagudo, Paula
author
Pina-Pedrero, Sonia
author
Rodríguez, Fernando
author
Moreno, Ángela
author
Garrido, Juan J.
other
Producció Animal
group
Sanitat Animal
2025-10-22T11:20:09Z
2025-10-22T11:20:09Z
2018-09-12
Herrera-Uribe, Júber, Ángeles Jiménez-Marín, Anna Lacasta, Paula L. Monteagudo, Sonia Pina-Pedrero, Fernando Rodríguez, Ángela Moreno, and Juan J. Garrido. 2018. "Comparative Proteomic Analysis Reveals Different Responses In Porcine Lymph Nodes To Virulent And Attenuated Homologous African Swine Fever Virus Strains". Veterinary Research 49 (1). Springer Nature America, Inc. doi:10.1186/s13567-018-0585-z.
0928-4249
http://hdl.handle.net/20.500.12327/180
https://doi.org/10.1186/s13567-018-0585-z
African swine fever (ASF) is a pathology of pigs against which there is no treatment or vaccine. Understanding the
equilibrium between innate and adaptive protective responses and immune pathology might contribute to the
development of strategies against ASFV. Here we compare, using a proteomic approach, the course of the in vivo
infection caused by two homologous strains: the virulent E75 and the attenuated E75CV1. Our results show a progres‑
sive loss of proteins by day 7 post-infection (pi) with E75, refecting tissue destruction. Many signal pathways were
afected by both infections but in diferent ways and extensions. Cytoskeletal remodelling and clathrin-endocytosis
were afected by both isolates, while a greater number of proteins involved on infammatory and immunological
pathways were altered by E75CV1. 14-3-3 mediated signalling, related to immunity and apoptosis, was inhibited
by both isolates. The implication of the Rho GTPases by E75CV1 throughout infection is also evident. Early events
refected the lack of E75 recognition by the immune system, an evasion strategy acquired by the virulent strains, and
signifcant changes at 7 days post-infection (dpi), coinciding with the peak of infection and the time of death. The
protein signature at day 31 pi with E75CV1 seems to refect events observed at 1 dpi, including the upregulation of
proteosomal subunits and molecules described as autoantigens (vimentin, HSPB1, enolase and lymphocyte cytosolic
protein 1), which allow the speculation that auto-antibodies could contribute to chronic ASFV infections. Therefore,
the use of proteomics could help understand ASFV pathogenesis and immune protection, opening new avenues for
future research.
eng
Attribution 4.0 International
Comparative proteomic analysis reveals different responses in porcine lymph nodes to virulent and attenuated homologous African swine fever virus strains
info:eu-repo/semantics/article