2026-04-17T16:21:17Zhttps://recercat.cat/oai/request
oai:recercat.cat:20.500.12327/17842025-10-22T11:03:55Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT author F. Del-Saz, Néstor author Iglesias-Sanchez, Ariadna author Alonso-Forn, David author López-Gómez, Miguel author Palma, Francisco author Clemente-Moreno, María José author Fernie, Alisdair R. author Ribas-Carbo, Miquel author Florez-Sarasa, Igor other Producció Vegetal group Genòmica i Biotecnologia 2025-10-22T11:03:55Z 2025-10-22T11:03:55Z 2022-05-17 Del-Saz, Néstor F., Ariadna Iglesias-Sanchez, David Alonso-Forn, Miguel López-Gómez, Francisco Palma, María José Clemente-Moreno, Alisdair R. Fernie, Miquel Ribas-Carbo, and Igor Florez-Sarasa. 2022. "The Lack Of Alternative Oxidase 1A Restricts In Vivo Respiratory Activity And Stress-Related Metabolism For Leaf Osmoprotection And Redox Balancing Under Sudden Acute Water And Salt Stress In Arabidopsis Thaliana". Frontiers In Plant Science 13. doi:10.3389/fpls.2022.833113. 1664-462X http://hdl.handle.net/20.500.12327/1784 https://doi.org/10.3389/fpls.2022.833113 In plants salt and water stress result in an induction of respiration and accumulation of stress-related metabolites (SRMs) with osmoregulation and osmoprotection functions that benefit photosynthesis. The synthesis of SRMs may depend on an active respiratory metabolism, which can be restricted under stress by the inhibition of the cytochrome oxidase pathway (COP), thus causing an increase in the reduction level of the ubiquinone pool. However, the activity of the alternative oxidase pathway (AOP) is thought to prevent this from occurring while at the same time, dissipates excess of reducing power from the chloroplast and thereby improves photosynthetic performance. The present research is based on the hypothesis that the accumulation of SRMs under osmotic stress will be affected by changes in folial AOP activity. To test this, the oxygen isotope-fractionation technique was used to study the in vivo respiratory activities of COP and AOP in leaves of wild-type Arabidopsis thaliana plants and of aox1a mutants under sudden acute stress conditions induced by mannitol and salt treatments. Levels of leaf primary metabolites and transcripts of respiratory-related proteins were also determined in parallel to photosynthetic analyses. The lack of in vivo AOP response in the aox1a mutants coincided with a lower leaf relative water content and a decreased accumulation of crucial osmoregulators. Additionally, levels of oxidative stress-related metabolites and transcripts encoding alternative respiratory components were increased. Coordinated changes in metabolite levels, respiratory activities and photosynthetic performance highlight the contribution of the AOP in providing flexibility to carbon metabolism for the accumulation of SRMs. eng Attribution 4.0 International The Lack of Alternative Oxidase 1a Restricts in vivo Respiratory Activity and Stress-Related Metabolism for Leaf Osmoprotection and Redox Balancing Under Sudden Acute Water and Salt Stress in Arabidopsis thaliana info:eu-repo/semantics/article