2026-04-14T04:58:27Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/17612025-10-22T11:25:11Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
López-Cano, Adrià
author
Bach, Alex
author
López-Serrano, Sergi
author
Aragon, Virginia
author
Blanch, Marta
author
Pastor, Jose J.
author
Tedó, Gemma
author
Morais, Sofia
author
Garcia-Fruitos, Elena
author
Alomar, Oscar
other
Producció Animal
group
Sanitat Animal
group
Producció de Remugants
group
Producció de Remugants
2025-10-22T11:25:11Z
2025-10-22T11:25:11Z
2022-04-21
López-Cano, Adrià, Alex Bach, Sergi López-Serrano, Virginia Aragon, Marta Blanch, Jose J. Pastor, Gemma Tedó, Sofia Morais, Elena Garcia-Fruitós, and Anna Arís. 2022. "Potential Of Oral Nanoparticles Containing Cytokines As Intestinal Mucosal Immunostimulants In Pigs: A Pilot Study". Animals 12 (9): 1075. doi:10.3390/ani12091075.
2076-2615
http://hdl.handle.net/20.500.12327/1761
https://doi.org/10.3390/ani12091075
Antimicrobial resistance is a global threat that is worryingly rising in the livestock sector.
Among the proposed strategies, immunostimulant development appears an interesting approach to
increase animal resilience at critical production points. The use of nanoparticles based on cytokine
aggregates, called inclusion bodies (IBs), has been demonstrated as a new source of immunostimulants
in aquaculture. Aiming to go a step further, the objective of this study was to produce cytokine
nanoparticles using a food-grade microorganism and to test their applicability to stimulate intestinal
mucosa in swine. Four cytokines (IL-1β, IL-6, IL-8, and TNF-α) involved in inflammatory response
were produced recombinantly in Lactococcus lactis in the form of protein nanoparticles (IBs). They
were able to stimulate inflammatory responses in a porcine enterocyte cell line (IPEC-J2) and alveolar
macrophages, maintaining high stability at low pH and high temperature. In addition, an in vivo
assay was conducted involving 20 piglets housed individually as a preliminary exploration of the
potential effects of IL-1β nanoparticles in piglet intestinal mucosa after a 7 d oral administration. The
treated animals tended to have greater levels of TNF-α in the blood, indicating that the tested dose of
nanoparticles tended to generate an inflammatory response in the animals. Whether this response is
sufficient to increase animal resilience needs further evaluation.
eng
Attribution 4.0 International
Potential of Oral Nanoparticles Containing Cytokines as Intestinal Mucosal Immunostimulants in Pigs: A Pilot Study
info:eu-repo/semantics/article